In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J. Sanchís-Benlloch; Josephine Nocillado; Claudia Ladisa; Joseph Aizen; Adam Miller; Michal Shpilman; Berta Levavi-Sivan; Tomer Ventura; Abigail Elizur

doi:10.1016/j.ygcen.2016.03.001

In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J. Sanchís-Benlloch, Josephine Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura, Abigail Elizur

Department of Animal Sciences

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E₂ and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E₂ levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

Original language	American English
Pages (from-to)	41-49
Number of pages	9
Journal	General and Comparative Endocrinology
Volume	241
DOIs	https://doi.org/10.1016/j.ygcen.2016.03.001
State	Published - 15 Jan 2017

Keywords

FSH
Pichia pastoris
Recombinant protein
Seriola lalandi
Teleost puberty

All Science Journal Classification (ASJC) codes

Animal Science and Zoology
Endocrinology

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10.1016/j.ygcen.2016.03.001

Cite this

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title = "In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone",

abstract = "Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.",

keywords = "FSH, Pichia pastoris, Recombinant protein, Seriola lalandi, Teleost puberty",

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doi = "10.1016/j.ygcen.2016.03.001",

language = "American English",

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T1 - In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

AU - Sanchís-Benlloch, Pablo J.

AU - Nocillado, Josephine

AU - Ladisa, Claudia

AU - Aizen, Joseph

AU - Miller, Adam

AU - Shpilman, Michal

AU - Levavi-Sivan, Berta

AU - Ventura, Tomer

AU - Elizur, Abigail

PY - 2017/1/15

Y1 - 2017/1/15

N2 - Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

AB - Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

KW - FSH

KW - Pichia pastoris

KW - Recombinant protein

KW - Seriola lalandi

KW - Teleost puberty

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U2 - 10.1016/j.ygcen.2016.03.001

DO - 10.1016/j.ygcen.2016.03.001

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SN - 0016-6480

VL - 241

SP - 41

EP - 49

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

ER -

In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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