In Situ Measurements of Cell Mechanical Properties Using Force Spectroscopy

Meital Reches, Tal Duanis-Assaf

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Mechanobiology focuses on how physical forces and the mechanical properties of cells and whole tissues affect their function. The mechanical properties of cells are of particular interest to developmental biology and stem cell differentiation, lymphocyte activation and phagocytic action in phagocytes, and development of malignant tumors and metastases. These properties can be measured on whole tissue and cell culture. Advances in instrument sensitivity and design, as well as improved techniques and scientific know-how achieved over the past few decades, allow researchers to study the mechanical properties of single cells and even at the subcellular level. Particularly, nanoindentation measurements using atomic force microscopy (AFM) mechanically probes single cells and even allows mapping of these traits. This chapter discusses these measurements from the experimental design to the analysis.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
Number of pages19
StatePublished - 2023

Publication series

NameMethods in Molecular Biology


  • Atomic force microscopy
  • Cell elasticity
  • Cell mechanics
  • Force spectroscopy
  • Nanoindentation
  • Single cell

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology


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