@article{86033a11c1df4adaa8693af8819ef551,
title = "In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning",
abstract = "Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called iridophores. It was suggested that the zebrafish{\textquoteright}s color pattern arises from a single type of iridophore migrating differentially to stripes and interstripes. However, here we find that iridophores do not migrate between stripes and interstripes but instead differentiate and proliferate in-place, based on their micro-environment. RNA-sequencing analysis further reveals that stripe and interstripe iridophores have different transcriptomic states, while cryogenic-scanning-electron-microscopy and micro-X-ray diffraction identify different crystal-arrays architectures, indicating that stripe and interstripe iridophores are different cell types. Based on these results, we present an alternative model of skin patterning in zebrafish in which distinct iridophore crystallotypes containing specialized, physiologically responsive, organelles arise in stripe and interstripe by in-situ differentiation.",
author = "Dvir Gur and Bain, \{Emily J.\} and Johnson, \{Kory R.\} and Aman, \{Andy J.\} and Pasoili, \{H. Amalia\} and Flynn, \{Jessica D.\} and Allen, \{Michael C.\} and Deheyn, \{Dimitri D.\} and Lee, \{Jennifer C.\} and Jennifer Lippincott-Schwartz and Parichy, \{David M.\}",
note = "We thank Gil Levkowitz, Dan Oron, and Ran Darshan for their help and advice, and Ivo Zizak for his help with the diffraction measurements at the μ-Spot beamline in BESSY II. We thank H.Z.B. for the allocation of synchrotron radiation beamtime. We thank Andrew Lemire, Kathy Schaefer, and Lihua Wang for their help and support. We thank members of the Lippincott-Schwartz and Parichy labs for their support. This work was supported by Howard Hughes Medical Institute to JLS and NIH R35 GM122471 to D.P. E.B. was additionally supported by NIH T32 HD007183, D.G. was additionally supported by the Human Frontiers Cross-Disciplinary Postdoctoral Fellowship LT000767/2018, the European Molecular Biology Organization Long-Term Fellowship and the Rothchild Fellowship. Hyperspectral analyses, M.C.A. and D.D.D. were supported by a Multidisciplinary University Research Initiative (MURI)—Melanin Grant, AFOSR FA9550-18-1-0142 (to D. D.D.). J.D.F. and J.C.L. are supported by the Intramural Research Program at NIH, NHLBI. D.G., E.B., D.D.D., J.C.L., D.P., and J.L.-S. designed research; D.G., E.B., A.J.A., A.P., J.D.F., and M.C.A., performed research; D.G., E.B., K.J., J.D.F., D.D.D., J.C.L., D.P., and J.L.-S. analyzed data; and D.G., E.B., D.P., and J.L.-S. wrote the paper.",
year = "2020",
month = dec,
day = "15",
doi = "10.1038/s41467-020-20088-1",
language = "الإنجليزيّة",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Research",
number = "1",
}