In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Iris Mironi-Harpaz; Lena Hazanov; Guy Engel; Dvir Yelin; Dror Seliktar

doi:10.1002/adma.201404185

In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Iris Mironi-Harpaz, Lena Hazanov, Guy Engel, Dvir Yelin, Dror Seliktar

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked "stiffer" and physically crosslinked "softer" regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.

Original language	English
Pages (from-to)	1933-+
Number of pages	6
Journal	Advanced Materials
Volume	27
Issue number	11
DOIs	https://doi.org/10.1002/adma.201404185
State	Published - 18 Mar 2015

Keywords

3D cell culture
free-radical polymerization
microrheology
microscopic photopatterning
reverse thermal gelation

All Science Journal Classification (ASJC) codes

Mechanics of Materials
Mechanical Engineering
General Materials Science

Access to Document

10.1002/adma.201404185

Cite this

@article{b21b7eab5e8f43209e6d99e4b078986e,

title = "In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography",

abstract = "Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked {"}stiffer{"} and physically crosslinked {"}softer{"} regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.",

keywords = "3D cell culture, free-radical polymerization, microrheology, microscopic photopatterning, reverse thermal gelation",

author = "Iris Mironi-Harpaz and Lena Hazanov and Guy Engel and Dvir Yelin and Dror Seliktar",

note = "Publisher Copyright: {\textcopyright} 2015 Wiley-VCH Verlag GmbH \& Co. KGaA.",

year = "2015",

month = mar,

day = "18",

doi = "10.1002/adma.201404185",

language = "الإنجليزيّة",

volume = "27",

pages = "1933--+",

journal = "Advanced Materials",

issn = "0935-9648",

publisher = "Wiley-Blackwell",

number = "11",

}

TY - JOUR

T1 - In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

AU - Mironi-Harpaz, Iris

AU - Hazanov, Lena

AU - Engel, Guy

AU - Yelin, Dvir

AU - Seliktar, Dror

PY - 2015/3/18

Y1 - 2015/3/18

N2 - Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked "stiffer" and physically crosslinked "softer" regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.

AB - Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked "stiffer" and physically crosslinked "softer" regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale.

KW - 3D cell culture

KW - free-radical polymerization

KW - microrheology

KW - microscopic photopatterning

KW - reverse thermal gelation

UR - http://www.scopus.com/inward/record.url?scp=84924869023&partnerID=8YFLogxK

U2 - 10.1002/adma.201404185

DO - 10.1002/adma.201404185

M3 - مقالة

SN - 0935-9648

VL - 27

SP - 1933-+

JO - Advanced Materials

JF - Advanced Materials

IS - 11

ER -

In-Situ Architectures Designed in 3D Cell-Laden Hydrogels Using Microscopic Laser Photolithography

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this