In planta colonization and role of T6SS in two rice Kosakonia endophytes

Susan Mosquito, Iris Bertani, Danilo Licastro, Stéphane Compant, Michael P. Myers, Estefanía Hinarejos, Asaf Levy, Vittorio Venturi

Research output: Contribution to journalArticlepeer-review

Abstract

Endophytes live inside plants and are often beneficial. Kosakonia is a novel bacterial genus that includes many diazotrophic plant-associated isolates. Plant-bacteria studies on two rice endophytic Kosakonia beneficial strains were performed, including comparative genomics, secretome profiling, in planta tests, and a field release trial. The strains are efficient rhizoplane and root endosphere colonizers and localized in the root cortex. Secretomics revealed 144 putative secreted proteins, including type VI secretory system (T6SS) proteins. A Kosakonia T6SS genomic knock-out mutant showed a significant decrease in rhizoplane and endosphere colonization ability. A field trial using rice seed inoculated with Kosakonia spp. showed no effect on plant growth promotion upon nitrogen stress and microbiome studies revealed that Kosakonia spp. were significantly more present in the inoculated rice. Comparative genomics indicated that several protein domains were enriched in plant-associated Kosakonia spp. This study highlights that Kosakonia is an important, recently classified genus involved in plant-bacteria interaction.

Original languageAmerican English
Pages (from-to)349-363
Number of pages15
JournalMolecular Plant-Microbe Interactions
Volume33
Issue number2
DOIs
StatePublished - 2020

Keywords

  • Endophytes
  • Genomics
  • Microscopy and imaging

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

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