@inproceedings{b43caef1aa9b4d55a1da64a20960e251,
title = "Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads",
abstract = "One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200{\texttrademark} system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.",
keywords = "Autofluorescence, Fluorescence-based immunoassays, Magnetic beads, Magnetic modulation biosensing, Photobleaching",
author = "Shira Roth and Orr Hadass and Meir Cohen and Jasenka Verbarg and Jennifer Wilsey and Amos Danielli",
note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Frontiers in Biological Detection: From Nanosensors to Systems XI 2019 ; Conference date: 03-02-2019 Through 04-02-2019",
year = "2019",
doi = "https://doi.org/10.1117/12.2507597",
language = "الإنجليزيّة",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Amos Danielli and Miller, {Benjamin L.} and Weiss, {Sharon M.}",
booktitle = "Frontiers in Biological Detection",
address = "الولايات المتّحدة",
}