@article{26bf393d884146da82a2ec65eb201e6d,
title = "Impaired cell-cell communication in the multicellular cyanobacterium Anabaena affects carbon uptake, photosynthesis, and the cell wall",
abstract = "Cell-cell communication is an essential attribute of multicellular organisms. The effects of perturbed communication were studied in septal protein mutants of the heterocyst-forming filamentous cyanobacterium Anabaena sp. PCC 7120 model organism. Strains bearing sepJ and sepJ/fraC/fraD deletions showed differences in growth, pigment absorption spectra, and spatial patterns of expression of the hetR gene encoding a heterocyst differentiation master regulator. Global changes in gene expression resulting from deletion of those genes were mapped by RNA sequencing analysis of wild-type and mutant strains, both under nitrogen-replete and nitrogen-poor conditions. The effects of sepJ and fraC/fraD deletions were non-additive, and perturbed cell-cell communication led to significant changes in global gene expression. Most significant effects, related to carbon metabolism, included increased expression of genes encoding carbon uptake systems and components of the photosynthetic apparatus, as well as decreased expression of genes encoding cell wall components related to heterocyst differentiation and to polysaccharide export.",
author = "Sergio Camargo and Dena Leshkowitz and Vicente Mariscal and Enrique Flores and Joel Stavans and Rinat Arbel-Goren",
note = "We thank Antonia Herrero for stimulating discussions and Shlomit Gilad for construction of libraries of cDNA and sequencing. Work in Seville was supported by grant BFU2017-88202-P from Plan Estatal de Investigaci{\'o}n Cient{\'i}fica y T{\'e}cnica y de Innovaci{\'o}n, Spain, co-financed by the European Regional Development Fund, to E.F. Work in Rehovot was supported by a Siegried and Irma Ullman Professorial Chair to J.S., and by the Minerva Stiftung http://www.minerva.mpg.de/ to J.S. Author contributions - Conceptualization R.A.-G., E.F., and J.S.; Methodology V.M., R.A.-G., and J.S.; Validation D.L., B.D., R.A.-G., S.C., and J.S.; Formal Analysis D.L., B.D., S.C., R.A.-G., E.F., and J.S.; Investigation R.A.G. and S.C.; Resources V.M., D.L., B.D., E.F., S.C., R.A.-G., and J.S.; Data Curation D.L., B.D., R.A.-G., and J.S.; Writing – Original Draft S.C., R.A.-G., and J.S.; Writing – Review & Editing S.C., D.L., B.D., E.F. R.A.-G., and J.S.; Visualization S.C., D.L., B.D., R.A.-G., and J.S.; Supervision E.F., R.A.-G., and J.S.; Project Administration R.A.-G. and J.S.; Funding Acquisition E.F. and J.S.",
year = "2021",
month = jan,
day = "22",
doi = "10.1016/j.isci.2020.101977",
language = "الإنجليزيّة",
volume = "24",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "1",
}