IL-10R polymorphisms are associated with very-early-onset ulcerative colitis

Christopher J. Moran, Thomas D. Walters, Cong Hui Guo, Subra Kugathasan, Christoph Klein, Dan Turner, Victorien M. Wolters, Robert H. Bandsma, Marialena Mouzaki, Mary Zachos, Jacob C. Langer, Ernest Cutz, Susanne M. Benseler, Chaim M. Roifman, Mark S. Silverberg, Anne M. Griffiths, Scott B. Snapper, Aleixo M. Muise

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Interleukin-10 (IL-10) signaling genes are attractive inflammatory bowel disease (IBD) candidate genes as IL-10 restricts intestinal inflammation, IL-10 polymorphisms have been associated with IBD in genome-wide association studies, and mutations in IL-10 and IL-10 receptor (IL-10R) genes have been reported in immunodeficient children with severe infantile-onset IBD. Our objective was to determine if IL-10R polymorphisms were associated with early-onset IBD (EO-IBD) and very-early-onset IBD (VEO-IBD). Methods: Candidate-gene analysis of IL10RA and IL10RB was performed after initial sequencing of an infantile onset-IBD patient identified a novel homozygous mutation. The discovery cohort included 188 EO-IBD subjects and 188 healthy subjects. Polymorphisms associated with IBD in the discovery cohort were genotyped in an independent validation cohort of 422 EO-IBD subjects and 480 healthy subjects. Results: We identified a homozygous, splice-site point mutation in IL10RA in an infantile-onset IBD patient causing a premature stop codon (P206X) and IL-10 insensitivity. IL10RA and IL10RB sequencing in the discovery cohort identified five IL10RA polymorphisms associated with ulcerative colitis (UC) and two IL10RB polymorphisms associated with Crohn's disease (CD). Of these polymorphisms, two IL10RA single nucleotide polymorphisms, rs2228054 and rs2228055, were associated with VEO-UC in the discovery cohort and replicated in an independent validation cohort (odds ratio [OR] 3.08, combined P = 2 × 10-4; and OR 2.93, P = 6 × 10-4, respectively). Conclusions: We identified IL10RA polymorphisms that confer risk for developing VEO-UC. Additionally, we identified the first splice site mutation in IL10RA resulting in infantile-onset IBD. This study expands the phenotype of IL10RA polymorphisms to include both severe arthritis and VEO-UC.

Original languageAmerican English
Pages (from-to)115-123
Number of pages9
JournalInflammatory Bowel Diseases
Volume19
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • IL-10
  • IL-10 receptor
  • Immunodeficiency
  • Inflammatory bowel disease

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Gastroenterology

Fingerprint

Dive into the research topics of 'IL-10R polymorphisms are associated with very-early-onset ulcerative colitis'. Together they form a unique fingerprint.

Cite this