IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa

Andrey Ethan Rubin; Klimenty Levkov; Osman Berk Usta; Martin Yarmush; Alexander Golberg

doi:https://doi.org/10.1007/s10439-019-02225-0

IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa

Andrey Ethan Rubin, Klimenty Levkov, Osman Berk Usta, Martin Yarmush, Alexander Golberg

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm ⁻¹ , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm ² area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.

Original language	English
Pages (from-to)	1314-1325
Number of pages	12
Journal	Annals of Biomedical Engineering
Volume	47
Issue number	5
DOIs	https://doi.org/10.1007/s10439-019-02225-0
State	Published - 15 May 2019

Keywords

Electroporation
IGBT
Multidrug-resistant bacteria
Pseudomonas aeruginosa PAO1
Pulsed electric field

All Science Journal Classification (ASJC) codes

Biomedical Engineering

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https://doi.org/10.1007/s10439-019-02225-0

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title = "IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa",

abstract = " Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.",

keywords = "Electroporation, IGBT, Multidrug-resistant bacteria, Pseudomonas aeruginosa PAO1, Pulsed electric field",

author = "Rubin, {Andrey Ethan} and Klimenty Levkov and Usta, {Osman Berk} and Martin Yarmush and Alexander Golberg",

note = "Publisher Copyright: {\textcopyright} 2019, Biomedical Engineering Society.",

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doi = "https://doi.org/10.1007/s10439-019-02225-0",

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T2 - Design and In Vitro Studies on Pseudomonas aeruginosa

AU - Rubin, Andrey Ethan

AU - Levkov, Klimenty

AU - Usta, Osman Berk

AU - Yarmush, Martin

AU - Golberg, Alexander

PY - 2019/5/15

Y1 - 2019/5/15

N2 - Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.

AB - Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.

KW - Electroporation

KW - IGBT

KW - Multidrug-resistant bacteria

KW - Pseudomonas aeruginosa PAO1

KW - Pulsed electric field

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SN - 0090-6964

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JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

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IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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