Identifying RNA editing sites in miRNAs by deep sequencing

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.

Original languageEnglish
Title of host publicationDeep Sequencing Data Analysis
PublisherSpringer Nature
Number of pages12
ISBN (Print)9781627035132
StatePublished - 2013

Publication series

NameMethods in Molecular Biology


  • A-to-I editing
  • Bioinformatics
  • Deep sequencing
  • Genomics
  • miRNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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