TY - JOUR
T1 - Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics
AU - Nagler, Adi
AU - Kalaora, Shelly
AU - Barbolin, Chaya
AU - Gangaev, Anastasia
AU - Ketelaars, Steven L.C
AU - Alon, Michal
AU - Pai, Joy
AU - Benedek, Gil
AU - Yahalom-Ronen, Yfat
AU - Erez, Noam
AU - Greenberg, Polina
AU - Yagel, Gal
AU - Peri, Aviyah
AU - Levin, Yishai
AU - Satpathy, Ansuman T
AU - Bar-Haim, Erez
AU - Paran, Nir
AU - Kvistborg, Pia
AU - Samuels, Yardena
N1 - Publisher Copyright: © 2021 The Author(s)
PY - 2021/6/29
Y1 - 2021/6/29
N2 - The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. We find 26 HLA-I peptides and 36 HLA class II (HLA-II) peptides. Among the identified peptides, some are shared between different cells and some are derived from out-of-frame open reading frames (ORFs). Seven of these peptides were previously shown to be immunogenic, and we identify two additional immunoreactive peptides by using HLA multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on presented viral-specific antigens that span several of the viral genes. [Display omitted] HLA peptidomics enable the identification of SARS-CoV-2-derived HLA peptides•SARS-CoV-2 peptides can be derived from canonical and non-canonical ORFs•Shared SARS-CoV-2 peptides are identified in different cell types•Several SARS-CoV-2 peptides are immunogenic Using HLA peptidomics, Nagler et al. identify SARS-CoV-2-derived peptides presented by highly prevalent HLA-I and HLA-II. Identified peptides are derived from various canonical and non-canonical viral ORFs, are shared between different cells, and are immunogenic. As the identified peptides are unique to SARS-CoV-2, they could potentially serve as future treatment targets.
AB - The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. We find 26 HLA-I peptides and 36 HLA class II (HLA-II) peptides. Among the identified peptides, some are shared between different cells and some are derived from out-of-frame open reading frames (ORFs). Seven of these peptides were previously shown to be immunogenic, and we identify two additional immunoreactive peptides by using HLA multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on presented viral-specific antigens that span several of the viral genes. [Display omitted] HLA peptidomics enable the identification of SARS-CoV-2-derived HLA peptides•SARS-CoV-2 peptides can be derived from canonical and non-canonical ORFs•Shared SARS-CoV-2 peptides are identified in different cell types•Several SARS-CoV-2 peptides are immunogenic Using HLA peptidomics, Nagler et al. identify SARS-CoV-2-derived peptides presented by highly prevalent HLA-I and HLA-II. Identified peptides are derived from various canonical and non-canonical viral ORFs, are shared between different cells, and are immunogenic. As the identified peptides are unique to SARS-CoV-2, they could potentially serve as future treatment targets.
UR - http://www.scopus.com/inward/record.url?scp=85108507587&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2021.109305
DO - 10.1016/j.celrep.2021.109305
M3 - مقالة
SN - 2211-1247
VL - 35
JO - Cell reports (Cambridge)
JF - Cell reports (Cambridge)
IS - 13
M1 - 109305
ER -