Ice-binding proteins and the applicability and limitations of the kinetic pinning model

Michael Chasnitsky, Ido Braslavsky

Research output: Contribution to journalArticlepeer-review

Abstract

Ice-binding proteins (IBPs) are unique molecules that bind to and are active on the interface between two phases of water: Ice and liquid water. This property allows them to affect ice growth in multiple ways: Shaping ice crystals, suppressing the freezing point, inhibiting recrystallization and promoting nucleation. Advances in the protein's production technologies make these proteins promising agents for medical applications among others.Here, we focus on a special class of IBPs that suppress freezing by causing thermal hysteresis (TH): Antifreeze proteins (AFPs). The kinetic pinning model describes the dynamics of a growing ice face with proteins binding to it, which eventually slow it down to a halt. We use the kinetic pinning model, with some adjustments made, to study the TH dependence on the solution's concentration of AFPs by fitting the model to published experimental data. We find this model describes the activity of (moderate) type III AFPs well, but is inadequate for the (hyperactive) Tenebrio molitor AFPs. We also find the engulfment resistance to be a key parameter, which depends on the protein's size. Finally, we explain intuitively how TH depends on the seeding time of the ice crystal in the protein solution. Using this insight, we explain the discrepancy in TH measurements between different assays. This article is part of the theme issue 'The physics and chemistry of ice: Scaffolding across scales, from the viability of life to the formation of planets'.

Original languageEnglish
Article number20180391
JournalPhilosophical transactions. Series A, Mathematical, physical, and engineering sciences
Volume377
Issue number2146
DOIs
StatePublished - 3 Jun 2019

Keywords

  • Antifreeze proteins
  • Freezing
  • Ice
  • Ice-binding proteins
  • Thermal hysteresis

All Science Journal Classification (ASJC) codes

  • General Engineering
  • General Physics and Astronomy
  • General Mathematics

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