TY - JOUR
T1 - Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia
AU - Gebresilassie, Araya
AU - Abbasi, Ibrahim
AU - Aklilu, Essayas
AU - Yared, Solomon
AU - Kirstein, Oscar David
AU - Moncaz, Aviad
AU - Tekie, Habte
AU - Balkew, Meshesha
AU - Warburg, Alon
AU - Hailu, Asrat
AU - Gebre-Michael, Teshome
N1 - Publisher Copyright: © 2015 Gebresilassie et al.; licensee BioMed Central.
PY - 2015/5/13
Y1 - 2015/5/13
N2 - Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.
AB - Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.
KW - Bloodmeal
KW - Host preference
KW - Phlebotomus orientalis
KW - Tahtay Adiyabo
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=84930675388&partnerID=8YFLogxK
U2 - https://doi.org/10.1186/s13071-015-0883-5
DO - https://doi.org/10.1186/s13071-015-0883-5
M3 - مقالة
C2 - 25963759
SN - 1756-3305
VL - 8
JO - Parasites and Vectors
JF - Parasites and Vectors
IS - 1
M1 - 270
ER -
