Holographic microrefractometer

Hagay Shpaisman; Bhaskar Jyoti Krishnatreya; David G. Grier

doi:https://doi.org/10.1063/1.4747168

Holographic microrefractometer

Hagay Shpaisman, Bhaskar Jyoti Krishnatreya, David G. Grier

Research output: Contribution to journal › Article › peer-review

Abstract

In-line holographic microscopy of micrometer-scale colloidal spheres yields heterodyne scattering patterns that may be interpreted with Lorenz-Mie theory to obtain precise time-resolved information on the refractive index of the suspending medium. We demonstrate this approach to spatially resolved refractometry with measurements on calibrated refractive index standards and use it to monitor chemical concentration in a microfluidic channel. Using commercially available colloidal spheres as probe particles and a standard video camera for detection yields values for the fluid's refractive index at the position of each probe particle in each holographic snapshot with a demonstrated resolution of 2 × 10 ^-3 refractive index units (RIU) and a potential resolution surpassing 10 ^-4 RIU. The combination of spatial resolution, temporal resolution, multi-point in situ access, and technical simplicity recommends this technique for cost-effective lab-on-a-chip applications.

Original language	English
Article number	091102
Journal	Applied Physics Letters
Volume	101
Issue number	9
DOIs	https://doi.org/10.1063/1.4747168
State	Published - 27 Aug 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

Access to Document

https://doi.org/10.1063/1.4747168

Cite this

@article{70f231c7d3e047b09e501954951c8bb5,

title = "Holographic microrefractometer",

abstract = "In-line holographic microscopy of micrometer-scale colloidal spheres yields heterodyne scattering patterns that may be interpreted with Lorenz-Mie theory to obtain precise time-resolved information on the refractive index of the suspending medium. We demonstrate this approach to spatially resolved refractometry with measurements on calibrated refractive index standards and use it to monitor chemical concentration in a microfluidic channel. Using commercially available colloidal spheres as probe particles and a standard video camera for detection yields values for the fluid's refractive index at the position of each probe particle in each holographic snapshot with a demonstrated resolution of 2 × 10 -3 refractive index units (RIU) and a potential resolution surpassing 10 -4 RIU. The combination of spatial resolution, temporal resolution, multi-point in situ access, and technical simplicity recommends this technique for cost-effective lab-on-a-chip applications.",

author = "Hagay Shpaisman and {Jyoti Krishnatreya}, Bhaskar and Grier, {David G.}",

note = "Funding Information: We acknowledge valuable conversations with Fook Chiong Cheong. This work was supported in part by the National Science Foundation through Award Number DMR-0922680 and also by a grant from Procter and Gamble.",

year = "2012",

month = aug,

day = "27",

doi = "https://doi.org/10.1063/1.4747168",

language = "الإنجليزيّة",

volume = "101",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics",

number = "9",

}

TY - JOUR

T1 - Holographic microrefractometer

AU - Shpaisman, Hagay

AU - Jyoti Krishnatreya, Bhaskar

AU - Grier, David G.

N1 - Funding Information: We acknowledge valuable conversations with Fook Chiong Cheong. This work was supported in part by the National Science Foundation through Award Number DMR-0922680 and also by a grant from Procter and Gamble.

PY - 2012/8/27

Y1 - 2012/8/27

N2 - In-line holographic microscopy of micrometer-scale colloidal spheres yields heterodyne scattering patterns that may be interpreted with Lorenz-Mie theory to obtain precise time-resolved information on the refractive index of the suspending medium. We demonstrate this approach to spatially resolved refractometry with measurements on calibrated refractive index standards and use it to monitor chemical concentration in a microfluidic channel. Using commercially available colloidal spheres as probe particles and a standard video camera for detection yields values for the fluid's refractive index at the position of each probe particle in each holographic snapshot with a demonstrated resolution of 2 × 10 -3 refractive index units (RIU) and a potential resolution surpassing 10 -4 RIU. The combination of spatial resolution, temporal resolution, multi-point in situ access, and technical simplicity recommends this technique for cost-effective lab-on-a-chip applications.

AB - In-line holographic microscopy of micrometer-scale colloidal spheres yields heterodyne scattering patterns that may be interpreted with Lorenz-Mie theory to obtain precise time-resolved information on the refractive index of the suspending medium. We demonstrate this approach to spatially resolved refractometry with measurements on calibrated refractive index standards and use it to monitor chemical concentration in a microfluidic channel. Using commercially available colloidal spheres as probe particles and a standard video camera for detection yields values for the fluid's refractive index at the position of each probe particle in each holographic snapshot with a demonstrated resolution of 2 × 10 -3 refractive index units (RIU) and a potential resolution surpassing 10 -4 RIU. The combination of spatial resolution, temporal resolution, multi-point in situ access, and technical simplicity recommends this technique for cost-effective lab-on-a-chip applications.

UR - http://www.scopus.com/inward/record.url?scp=84865846774&partnerID=8YFLogxK

U2 - https://doi.org/10.1063/1.4747168

DO - https://doi.org/10.1063/1.4747168

M3 - مقالة

SN - 0003-6951

VL - 101

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 9

M1 - 091102

ER -

Holographic microrefractometer

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this