TY - JOUR
T1 - Highly Resolved Systems Biology to Dissect the Etioplast-to-Chloroplast Transition in Tobacco Leaves
AU - Armarego-Marriott, Tegan
AU - Kowalewska, Lucja
AU - Burgos, Asdrubal
AU - Fischer, Axel
AU - Thiele, Wolfram
AU - Erban, Alexander
AU - Strand, Deserah
AU - Kahlau, Sabine
AU - Hertle, Alexander
AU - Kopka, Joachim
AU - Walther, Dirk
AU - Reich, Ziv
AU - Schoettler, Mark Aurel
AU - Bock, Ralph
N1 - We thank the Max Planck Institute of Molecular Plant Physiology (MPI-MP) GreenTeam for help with plant cultivation and Dr. Michael Tillich (MPI-MP) for helpful discussions regarding RNAseq data. We thank Ines Fehrle and Änne Michaelis (MPI-MP) for excellent technical assistance. This work was supported by the Human Frontier Science Program to R.B. and Z.R. (RGP0005/2013). T.A-M., R.B., M.A.S., and Z.R. designed experiments; T.A -M. coordinated theproject; T.A-M., A.H., A.B., W.T., M.A.S., D.S., and Ł.K. performed experiments; T.A-M., Ł.K., A.E., J.K., A.F., M.A.S., and D.W. processed data; S.K. designed the qPCR platform; T.A-M. wrote the article with significant contribution from R.B., J.K., and M.A.S.; all authors contributed to the editing and review of the article.
PY - 2019/5
Y1 - 2019/5
N2 - Upon exposure to light, plant cells quickly acquire photosynthetic competence by converting pale etioplasts into green chloroplasts. This developmental transition involves the de novo biogenesis of the thylakoid system and requires reprogramming of metabolism and gene expression. Etioplast-to-chloroplast differentiation involves massive changes in plastid ultrastructure, but how these changes are connected to specific changes in physiology, metabolism, and expression of the plastid and nuclear genomes is poorly understood. Here, we describe a new experimental system in the dicotyledonous model plant tobacco (Nicotiana tabacum) that allows us to study the leaf deetiolation process at the systems level. We have determined the accumulation kinetics of photosynthetic complexes, pigments, lipids, and soluble metabolites and recorded the dynamic changes in plastid ultrastructure and in the nuclear and plastid transcriptomes. Our data describe the greening process at high temporal resolution, resolve distinct genetic and metabolic phases during deetiolation, and reveal numerous candidate genes that may be involved in light-induced chloroplast development and thylakoid biogenesis.
AB - Upon exposure to light, plant cells quickly acquire photosynthetic competence by converting pale etioplasts into green chloroplasts. This developmental transition involves the de novo biogenesis of the thylakoid system and requires reprogramming of metabolism and gene expression. Etioplast-to-chloroplast differentiation involves massive changes in plastid ultrastructure, but how these changes are connected to specific changes in physiology, metabolism, and expression of the plastid and nuclear genomes is poorly understood. Here, we describe a new experimental system in the dicotyledonous model plant tobacco (Nicotiana tabacum) that allows us to study the leaf deetiolation process at the systems level. We have determined the accumulation kinetics of photosynthetic complexes, pigments, lipids, and soluble metabolites and recorded the dynamic changes in plastid ultrastructure and in the nuclear and plastid transcriptomes. Our data describe the greening process at high temporal resolution, resolve distinct genetic and metabolic phases during deetiolation, and reveal numerous candidate genes that may be involved in light-induced chloroplast development and thylakoid biogenesis.
UR - https://www.scopus.com/pages/publications/85065662292
U2 - 10.1104/pp.18.01432
DO - 10.1104/pp.18.01432
M3 - Article
SN - 0032-0889
VL - 180
SP - 654
EP - 681
JO - Plant Physiology
JF - Plant Physiology
IS - 1
ER -