High-throughput single-molecule optofluidic analysis

Soohong Kim; Aaron M. Streets; Ron R. Lin; Stephen R. Quake; Shimon Weiss; Devdoot S. Majumdar

doi:10.1038/nmeth.1569

High-throughput single-molecule optofluidic analysis

Soohong Kim
, Aaron M. Streets
, Ron R. Lin
, Stephen R. Quake
, Shimon Weiss
, Devdoot S. Majumdar

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

Original language	English
Pages (from-to)	242-245
Number of pages	4
Journal	Nature Methods
Volume	8
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1569
State	Published - Mar 2011
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1569

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title = "High-throughput single-molecule optofluidic analysis",

abstract = "We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.",

author = "Soohong Kim and Streets, \{Aaron M.\} and Lin, \{Ron R.\} and Quake, \{Stephen R.\} and Shimon Weiss and Majumdar, \{Devdoot S.\}",

note = "Funding Information: We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship.",

year = "2011",

month = mar,

doi = "10.1038/nmeth.1569",

language = "English",

volume = "8",

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journal = "Nature Methods",

issn = "1548-7091",

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AU - Streets, Aaron M.

AU - Lin, Ron R.

AU - Quake, Stephen R.

AU - Weiss, Shimon

AU - Majumdar, Devdoot S.

N1 - Funding Information: We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship.

PY - 2011/3

Y1 - 2011/3

N2 - We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

AB - We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

UR - https://www.scopus.com/pages/publications/79952132910

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DO - 10.1038/nmeth.1569

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SN - 1548-7091

VL - 8

SP - 242

EP - 245

JO - Nature Methods

JF - Nature Methods

IS - 3

ER -

High-throughput single-molecule optofluidic analysis

Abstract

ASJC Scopus subject areas

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