@article{748adacc2fff47d292231f57d11323b4,
title = "High-throughput single-molecule optofluidic analysis",
abstract = "We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.",
author = "Soohong Kim and Streets, {Aaron M.} and Lin, {Ron R.} and Quake, {Stephen R.} and Shimon Weiss and Majumdar, {Devdoot S.}",
note = "Funding Information: We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship.",
year = "2011",
month = mar,
doi = "https://doi.org/10.1038/nmeth.1569",
language = "الإنجليزيّة",
volume = "8",
pages = "242--245",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "3",
}