High-throughput detection of RNA modifications at single base resolution

Keren Ron, Joshua Kahn, Nofar Malka-Tunitsky, Aldema Sas-Chen

Research output: Contribution to journalReview articlepeer-review

Abstract

RNA is modified by > 170 chemical modifications that affect its structure and function. Accordingly, RNA modifications have been implicated in regulation of gene expression and cellular outcomes in a variety of species spanning the phylogenetic tree. The study of RNA modifications is accelerated by generation of high-throughput methods for detecting RNA modifications at single base resolution. Here, we review recent advancement in next generation sequencing based approaches for detection of 14 distinct RNA modifications present in rRNA, tRNA and mRNA. We further outline the molecular and computational principles underlying currently available methods.

Original languageEnglish
Pages (from-to)19-32
Number of pages14
JournalFEBS Letters
Volume599
Issue number1
DOIs
StatePublished - Jan 2025

Keywords

  • RNA modifications
  • epitranscriptome
  • mass-spectrometry
  • nanopore
  • next-generation sequencing

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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