High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

Ying Ma, Lin Ma, Juanjuan Zheng, Min Liu, Zeev Zalevsky, Peng Gao

Research output: Contribution to journalArticlepeer-review

Abstract

Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system’s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.

Original languageEnglish
Article number892529
JournalFrontiers in Physics
Volume10
DOIs
StatePublished - 9 May 2022

Keywords

  • label-free
  • non-invasive
  • phase contrast
  • quantitative phase imaging
  • ultra-oblique illumination

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Materials Science (miscellaneous)
  • Mathematical Physics
  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Fingerprint

Dive into the research topics of 'High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy'. Together they form a unique fingerprint.

Cite this