Abstract
Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system’s structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, π/2, π, and 3π/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.
Original language | English |
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Article number | 892529 |
Journal | Frontiers in Physics |
Volume | 10 |
DOIs | |
State | Published - 9 May 2022 |
Keywords
- label-free
- non-invasive
- phase contrast
- quantitative phase imaging
- ultra-oblique illumination
All Science Journal Classification (ASJC) codes
- Biophysics
- General Physics and Astronomy
- Mathematical Physics
- Physical and Theoretical Chemistry
- Materials Science (miscellaneous)