High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

Itay Remer; Roni Shaashoua; Netta Shemesh; Anat Ben-Zvi; Alberto Bilenca

doi:10.1038/s41592-020-0882-0

High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

Itay Remer, Roni Shaashoua, Netta Shemesh, Anat Ben-Zvi, Alberto Bilenca

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.

Original language	American English
Pages (from-to)	913-916
Number of pages	4
Journal	Nature Methods
Volume	17
Issue number	9
DOIs	https://doi.org/10.1038/s41592-020-0882-0
State	Published - 1 Sep 2020

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.",

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AU - Bilenca, Alberto

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AB - Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.

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High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

Abstract

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