@inbook{45b3e85313234fafbe4253d8b6f5e406,
title = "High-Resolution scanning electron microscopy for the imaging of nuclear pore complexes and ran-mediated transport",
abstract = "High-resolution scanning electron microscopy provides three-dimensional surface images of nuclear pore complexes (NPCs) embedded in the nuclear envelope. Here, we describe a method for exposing the nuclear surface in mammalian tissue culture cells for imaging by scanning electron microscopy. Hypotonic treatment is followed by low-speed centrifugation onto polylysine-coated silicon chips, without the use of detergents. This helps to preserve NPCs close to their native morphology, embedded in undamaged nuclear membranes. This method is particularly advantageous for combining high-resolution imaging of NPCs with mammalian genetic systems.",
keywords = "Mammalian cell culture, Nuclear envelope, Nuclear pore complex, Scanning electron microscopy",
author = "Lihi Shaulov and Boris Fichtman and Amnon Harel",
year = "2014",
doi = "10.1007/978-1-62703-791-4\_17",
language = "الإنجليزيّة",
isbn = "9781627037907",
series = "Methods in Molecular Biology",
pages = "253--261",
booktitle = "Ras Signaling",
}