High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Boris Fichtman; Saroj G. Regmi; Mary Dasso; Amnon Harel

doi:10.1007/978-1-0716-2337-4_29

High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Boris Fichtman
, Saroj G. Regmi
, Mary Dasso
, Amnon Harel

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	461-471
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-2337-4_29
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2502

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Auxin-inducible degron
Central channel particles
Mammalian cell culture
Nuclear envelope
Nuclear pore complex
Scanning electron microscopy

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-2337-4_29

Cite this

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title = "High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes",

abstract = "Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.",

keywords = "Auxin-inducible degron, Central channel particles, Mammalian cell culture, Nuclear envelope, Nuclear pore complex, Scanning electron microscopy",

author = "Boris Fichtman and Regmi, \{Saroj G.\} and Mary Dasso and Amnon Harel",

note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2337-4\_29",

language = "English",

series = "Methods in Molecular Biology",

pages = "461--471",

booktitle = "Methods in Molecular Biology",

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TY - CHAP

T1 - High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

AU - Fichtman, Boris

AU - Regmi, Saroj G.

AU - Dasso, Mary

AU - Harel, Amnon

PY - 2022

Y1 - 2022

N2 - Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.

AB - Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.

KW - Auxin-inducible degron

KW - Central channel particles

KW - Mammalian cell culture

KW - Nuclear envelope

KW - Nuclear pore complex

KW - Scanning electron microscopy

UR - https://www.scopus.com/pages/publications/85128010512

U2 - 10.1007/978-1-0716-2337-4_29

DO - 10.1007/978-1-0716-2337-4_29

M3 - Chapter

C2 - 35412256

T3 - Methods in Molecular Biology

SP - 461

EP - 471

BT - Methods in Molecular Biology

ER -

High-Resolution Imaging and Analysis of Individual Nuclear Pore Complexes

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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