High-power near-concentric Fabry–Perot cavity for phase contrast electron microscopy
Carter Turnbaugh, Jeremy J Axelrod, Sara L Campbell, Jeske Y Dioquino, Petar N Petrov, Jonathan Remis, Osip Schwartz, Zanlin Yu, Yifan Cheng, Robert M Glaeser, Holger Mueller
Research output: Contribution to journal › Article › peer-review
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry–Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.