High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy

Carter Turnbaugh; Jeremy J Axelrod; Sara L Campbell; Jeske Y Dioquino; Petar N Petrov; Jonathan Remis; Osip Schwartz; Zanlin Yu; Yifan Cheng; Robert M Glaeser; Holger Mueller

doi:https://doi.org/10.1063/5.0045496

High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy

Carter Turnbaugh, Jeremy J Axelrod, Sara L Campbell, Jeske Y Dioquino, Petar N Petrov, Jonathan Remis, Osip Schwartz, Zanlin Yu, Yifan Cheng, Robert M Glaeser, Holger Mueller

Department of Physics of Complex Systems

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.

Original language	English
Article number	053005
Journal	Review of Scientific Instruments
Volume	92
Issue number	5
DOIs	https://doi.org/10.1063/5.0045496
State	Published - 1 May 2021

All Science Journal Classification (ASJC) codes

Instrumentation

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https://doi.org/10.1063/5.0045496License: CC BY

Cite this

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title = "High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy",

abstract = "Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.",

author = "Carter Turnbaugh and Axelrod, {Jeremy J} and Campbell, {Sara L} and Dioquino, {Jeske Y} and Petrov, {Petar N} and Jonathan Remis and Osip Schwartz and Zanlin Yu and Yifan Cheng and Glaeser, {Robert M} and Holger Mueller",

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doi = "https://doi.org/10.1063/5.0045496",

language = "الإنجليزيّة",

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TY - JOUR

T1 - High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy

AU - Turnbaugh, Carter

AU - Axelrod, Jeremy J

AU - Campbell, Sara L

AU - Dioquino, Jeske Y

AU - Petrov, Petar N

AU - Remis, Jonathan

AU - Schwartz, Osip

AU - Yu, Zanlin

AU - Cheng, Yifan

AU - Glaeser, Robert M

AU - Mueller, Holger

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.

AB - Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry-Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.

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DO - https://doi.org/10.1063/5.0045496

M3 - مقالة

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SN - 0034-6748

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JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

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M1 - 053005

ER -

High-power near-concentric Fabry-Perot cavity for phase contrast electron microscopy

Abstract

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