High frame-rate resolution of cell division during Candida albicans filamentation

Darren D. Thomson; Judith Berman; Alexandra C. Brand

doi:10.1016/j.fgb.2016.02.001

High frame-rate resolution of cell division during Candida albicans filamentation

Darren D. Thomson, Judith Berman, Alexandra C. Brand

Biotechnology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

Original language	English
Pages (from-to)	54-58
Number of pages	5
Journal	Fungal Genetics and Biology
Volume	88
DOIs	https://doi.org/10.1016/j.fgb.2016.02.001
State	Published - 1 Mar 2016

Keywords

Hypha
Nuclear division
Septum

All Science Journal Classification (ASJC) codes

Microbiology
Genetics

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10.1016/j.fgb.2016.02.001

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title = "High frame-rate resolution of cell division during Candida albicans filamentation",

abstract = "The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.",

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AU - Thomson, Darren D.

AU - Berman, Judith

AU - Brand, Alexandra C.

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Y1 - 2016/3/1

N2 - The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

AB - The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

KW - Hypha

KW - Nuclear division

KW - Septum

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U2 - 10.1016/j.fgb.2016.02.001

DO - 10.1016/j.fgb.2016.02.001

M3 - مقالة

SN - 1087-1845

VL - 88

SP - 54

EP - 58

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -

High frame-rate resolution of cell division during Candida albicans filamentation

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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