@inbook{2704e121e6ee45c8988ad661249c8acd,
title = "High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging",
abstract = "Multiplexed Ion Beam Imaging by Time of Flight (MIBI-TOF) enables high-dimensional imaging in situ of clinical specimens at single-cell resolution. In MIBI-TOF, tissue sections are stained with dozens of metal-labeled antibodies, whose abundance and location are read by secondary ionization mass spectrometry. The result is a multi-dimensional image, depicting sub-cellular expression and localization for dozens of distinct proteins in situ. Here, we describe the staining and imaging procedures of a MIBI-TOF experiment.",
author = "Ofer Elhanani and Leeat Keren and Michael Angelo",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "https://doi.org/10.1007/978-1-0716-1771-7_10",
language = "الإنجليزيّة",
isbn = "1071617702",
series = "Methods in Molecular Biology",
publisher = "Springer New York",
pages = "147--156",
booktitle = "Single-Cell Protein Analysis",
address = "الولايات المتّحدة",
}