High- and Super-Resolution Imaging of Cell-Cell Interfaces

Julia Sajman, Eilon Sherman

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Physical interfaces mediate interactions between multiple types of cells. Despite the importance of such interfaces to the cells’ function, their high-resolution optical imaging has been typically limited due to poor alignment of the interfaces relative to the optical plane of imaging. Here, we present a simple and robust method to align cell-cell interfaces in parallel to the coverslip by adhering the interacting cells to two opposing coverslips and bringing them into contact in a controlled and stable fashion. We demonstrate aberration-free high-resolution imaging of interfaces between live T cells and antigen-presenting cells, known as immune synapses, as an outstanding example. Imaging methods may include multiple diffraction-limited and super-resolution microscopy techniques (e.g., bright-field, confocal, STED, and dSTORM). Thus, our simple and widely compatible approach allows imaging with high- and super-resolution the intricate structure and molecular organization within a variety of cell-cell interfaces.

Original languageAmerican English
Title of host publicationThe Immune Synapse
EditorsCosima T. Baldari, Michael L. Dustin
Pages149-158
Number of pages10
ISBN (Electronic)978-1-0716-3135-5
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2654

Keywords

  • Cell-to-cell interaction
  • Diffraction limit
  • Imaging
  • Immune synapse
  • Microcopy
  • Super-resolution
  • T cells

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'High- and Super-Resolution Imaging of Cell-Cell Interfaces'. Together they form a unique fingerprint.

Cite this