Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Ari E. Friedland; Yonatan B. Tzur; Kevin M. Esvelt; Monica P. Colaiácovo; George M. Church; John A. Calarco

doi:10.1038/nmeth.2532

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Ari E. Friedland, Yonatan B. Tzur, Kevin M. Esvelt, Monica P. Colaiácovo, George M. Church, John A. Calarco

Research output: Contribution to journal › Article › peer-review

Abstract

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

Original language	English
Pages (from-to)	741-743
Number of pages	3
Journal	Nature Methods
Volume	10
Issue number	8
DOIs	https://doi.org/10.1038/nmeth.2532
State	Published - Aug 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.2532

Cite this

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title = "Heritable genome editing in C. elegans via a CRISPR-Cas9 system",

abstract = "We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.",

author = "Friedland, \{Ari E.\} and Tzur, \{Yonatan B.\} and Esvelt, \{Kevin M.\} and Colai{\'a}covo, \{Monica P.\} and Church, \{George M.\} and Calarco, \{John A.\}",

note = "Funding Information: We thank members of the Caenorhabditis Genetics Center for providing the N2 strain used in our experiments, and B. Stern, A. Murray, A. Saltzman, Joe Calarco and members of the Calarco laboratory for comments on the manuscript. This work was supported by US National Institutes of Health Early Independence Award (1DP5OD009153) and additional support from Harvard University to J.A.C., by National Institutes of Health grant R01GM072551 to M.P.C., and a National Human Genome Research Institute Center of Excellence in Genome Sciences award to G.M.C. A.E.F. is supported by a Ralph Ellison/American Federation for Aging Research postdoctoral fellowship.",

year = "2013",

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doi = "10.1038/nmeth.2532",

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volume = "10",

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T1 - Heritable genome editing in C. elegans via a CRISPR-Cas9 system

AU - Friedland, Ari E.

AU - Tzur, Yonatan B.

AU - Esvelt, Kevin M.

AU - Colaiácovo, Monica P.

AU - Church, George M.

AU - Calarco, John A.

N1 - Funding Information: We thank members of the Caenorhabditis Genetics Center for providing the N2 strain used in our experiments, and B. Stern, A. Murray, A. Saltzman, Joe Calarco and members of the Calarco laboratory for comments on the manuscript. This work was supported by US National Institutes of Health Early Independence Award (1DP5OD009153) and additional support from Harvard University to J.A.C., by National Institutes of Health grant R01GM072551 to M.P.C., and a National Human Genome Research Institute Center of Excellence in Genome Sciences award to G.M.C. A.E.F. is supported by a Ralph Ellison/American Federation for Aging Research postdoctoral fellowship.

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AB - We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

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U2 - 10.1038/nmeth.2532

DO - 10.1038/nmeth.2532

M3 - مقالة

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SP - 741

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JO - Nature Methods

JF - Nature Methods

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ER -

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Abstract

All Science Journal Classification (ASJC) codes

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