Hemodynamic and morphologic responses in mouse brain during acute head injury imaged by multispectral structured illumination

Boris Volkov, Marlon S. Mathews, David Abookasis

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Multispectral imaging has received significant attention over the last decade as it integrates spectroscopy, imaging, tomography analysis concurrently to acquire both spatial and spectral information from biological tissue. In the present study, a multispectral setup based on projection of structured illumination at several near-infrared wavelengths and at different spatial frequencies is applied to quantitatively assess brain function before, during, and after the onset of traumatic brain injury in an intact mouse brain (n=5). For the production of head injury, we used the weight drop method where weight of a cylindrical metallic rod falling along a metal tube strikes the mouse's head. Structured light was projected onto the scalp surface and diffuse reflected light was recorded by a CCD camera positioned perpendicular to the mouse head. Following data analysis, we were able to concurrently show a series of hemodynamic and morphologic changes over time including higher deoxyhemoglobin, reduction in oxygen saturation, cell swelling, etc., in comparison with baseline measurements. Overall, results demonstrates the capability of multispectral imaging based structured illumination to detect and map of brain tissue optical and physiological properties following brain injury in a simple noninvasive and noncontact manner.

Original languageEnglish
Title of host publicationOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
EditorsHenry Hirschberg, Steen J. Madsen, Qingming Luo, Samarendra K. Mohanty, Nitish V. Thakor, E. Duco Jansen
PublisherSPIE
ISBN (Electronic)9781628413953
DOIs
StatePublished - 2015
EventOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II - San Francisco, United States
Duration: 7 Feb 201510 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9305

Conference

ConferenceOptical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Country/TerritoryUnited States
CitySan Francisco
Period7/02/1510/02/15

Keywords

  • Cerebral hemodynamics
  • Diffuse reflectance
  • Head injury
  • Multispectral imaging
  • Optical properties
  • Structured illumination

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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