TY - JOUR
T1 - Gonadotropic and physiological functions of juvenile hormone in bumblebee (Bombus terrestris) workers
AU - Shpigler, Hagai
AU - Amsalem, Etya
AU - Huang, Zachary Y.
AU - Cohen, Mira
AU - Siegel, Adam J.
AU - Hefetz, Abraham
AU - Bloch, Guy
N1 - Funding Information: We thank Yafit Brener for assistance with data collection, Nadav Yayon for manufacturing the surgical table, Alex Kutovoy for help with bioinformatics and Gro V. Amdam for providing the anti-AmVG antibody. HS was supported by a "Hoffman Leadership and Responsibility" doctoral scholarship, and the Vaadia-BARD Postdoctoral Fellowship Award No. FI-462-2012 from BARD.
PY - 2014/6/24
Y1 - 2014/6/24
N2 - The evolution of advanced sociality in bees is associated with apparent modifications in juvenile hormone (JH) signaling. By contrast to most insects in which JH is a gonadotropin regulating female fertility, in the highly eusocial honey bee (Apis mellifera) JH has lost its gonadotrophic function in adult females, and instead regulates age-related division of labor among worker bees. In order to shed light on the evolution of JH signaling in bees we performed allatectomy and replacement therapies to manipulate JH levels in workers of the "primitively eusocial" bumblebee Bombus terrestris. Allatectomized worker bees showed remarkable reduction in ovarian development, egg laying, Vitellogenin and Krüppel homolog 1 fat body transcript levels, hemolymph Vitellogenin protein abundance, wax secretion, and egg-cell construction. These effects were reverted, at least partially, by treating allatectomized bees with JH-III, the natural JH of bees. Allatectomy also affected the amount of ester component in Dufour's gland secretion, which is thought to convey a social signal relating to worker fertility. These findings provide a strong support for the hypothesis that in contrast to honey bees, JH is a gonadotropin in bumblebees and lend credence to the hypothesis that the evolution of advanced eusociality in honey bees was associated with major modifications in JH signaling.
AB - The evolution of advanced sociality in bees is associated with apparent modifications in juvenile hormone (JH) signaling. By contrast to most insects in which JH is a gonadotropin regulating female fertility, in the highly eusocial honey bee (Apis mellifera) JH has lost its gonadotrophic function in adult females, and instead regulates age-related division of labor among worker bees. In order to shed light on the evolution of JH signaling in bees we performed allatectomy and replacement therapies to manipulate JH levels in workers of the "primitively eusocial" bumblebee Bombus terrestris. Allatectomized worker bees showed remarkable reduction in ovarian development, egg laying, Vitellogenin and Krüppel homolog 1 fat body transcript levels, hemolymph Vitellogenin protein abundance, wax secretion, and egg-cell construction. These effects were reverted, at least partially, by treating allatectomized bees with JH-III, the natural JH of bees. Allatectomy also affected the amount of ester component in Dufour's gland secretion, which is thought to convey a social signal relating to worker fertility. These findings provide a strong support for the hypothesis that in contrast to honey bees, JH is a gonadotropin in bumblebees and lend credence to the hypothesis that the evolution of advanced eusociality in honey bees was associated with major modifications in JH signaling.
UR - http://www.scopus.com/inward/record.url?scp=84903530863&partnerID=8YFLogxK
U2 - https://doi.org/10.1371/journal.pone.0100650
DO - https://doi.org/10.1371/journal.pone.0100650
M3 - مقالة
C2 - 24959888
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 6
M1 - e100650
ER -