Genome-wide SWAp-Tag yeast libraries for proteome exploration

Uri Weill; Ido Yofe; Ehud Sass; Bram Stynen; Dan Davidi; Janani Natarajan; Reut Ben-Menachem; Zohar Avihou; Omer Goldman; Nofar Harpaz; Silvia Chuartzman; Kiril Kniazev; Barbara Knoblach; Janina Laborenz; Felix Boos; Jacqueline Kowarzyk; Shifra Ben-Dor; Einat Zalckvar; Johannes M. Herrmann; Richard A. Rachubinski; Ophry Pines; Doron Rapaport; Stephen W. Michnick; Emmanuel D. Levy; Maya Schuldiner

doi:https://doi.org/10.1038/s41592-018-0044-9

Genome-wide SWAp-Tag yeast libraries for proteome exploration

Uri Weill, Ido Yofe, Ehud Sass, Bram Stynen, Dan Davidi, Janani Natarajan, Reut Ben-Menachem, Zohar Avihou, Omer Goldman, Nofar Harpaz, Silvia Chuartzman, Kiril Kniazev, Barbara Knoblach, Janina Laborenz, Felix Boos, Jacqueline Kowarzyk, Shifra Ben-Dor, Einat Zalckvar, Johannes M. Herrmann, Richard A. RachubinskiOphry Pines, Doron Rapaport, Stephen W. Michnick, Emmanuel D. Levy, Maya Schuldiner

Weizmann Institute of Science, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

Original language	English
Pages (from-to)	617-622
Number of pages	6
Journal	Nature Methods
Volume	15
Issue number	8
DOIs	https://doi.org/10.1038/s41592-018-0044-9
State	Published - 1 Aug 2018

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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https://doi.org/10.1038/s41592-018-0044-9

Cite this

Weill, U., Yofe, I., Sass, E., Stynen, B., Davidi, D., Natarajan, J., Ben-Menachem, R., Avihou, Z., Goldman, O., Harpaz, N., Chuartzman, S., Kniazev, K., Knoblach, B., Laborenz, J., Boos, F., Kowarzyk, J., Ben-Dor, S., Zalckvar, E., Herrmann, J. M., ... Schuldiner, M. (2018). Genome-wide SWAp-Tag yeast libraries for proteome exploration. Nature Methods, 15(8), 617-622. https://doi.org/10.1038/s41592-018-0044-9

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title = "Genome-wide SWAp-Tag yeast libraries for proteome exploration",

abstract = "Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.",

author = "Uri Weill and Ido Yofe and Ehud Sass and Bram Stynen and Dan Davidi and Janani Natarajan and Reut Ben-Menachem and Zohar Avihou and Omer Goldman and Nofar Harpaz and Silvia Chuartzman and Kiril Kniazev and Barbara Knoblach and Janina Laborenz and Felix Boos and Jacqueline Kowarzyk and Shifra Ben-Dor and Einat Zalckvar and Herrmann, {Johannes M.} and Rachubinski, {Richard A.} and Ophry Pines and Doron Rapaport and Michnick, {Stephen W.} and Levy, {Emmanuel D.} and Maya Schuldiner",

note = "Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

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doi = "https://doi.org/10.1038/s41592-018-0044-9",

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Weill, U, Yofe, I, Sass, E, Stynen, B, Davidi, D, Natarajan, J, Ben-Menachem, R, Avihou, Z, Goldman, O, Harpaz, N, Chuartzman, S, Kniazev, K, Knoblach, B, Laborenz, J, Boos, F, Kowarzyk, J, Ben-Dor, S, Zalckvar, E, Herrmann, JM, Rachubinski, RA, Pines, O, Rapaport, D, Michnick, SW, Levy, ED & Schuldiner, M 2018, 'Genome-wide SWAp-Tag yeast libraries for proteome exploration', Nature Methods, vol. 15, no. 8, pp. 617-622. https://doi.org/10.1038/s41592-018-0044-9

TY - JOUR

T1 - Genome-wide SWAp-Tag yeast libraries for proteome exploration

AU - Weill, Uri

AU - Yofe, Ido

AU - Sass, Ehud

AU - Stynen, Bram

AU - Davidi, Dan

AU - Natarajan, Janani

AU - Ben-Menachem, Reut

AU - Avihou, Zohar

AU - Goldman, Omer

AU - Harpaz, Nofar

AU - Chuartzman, Silvia

AU - Kniazev, Kiril

AU - Knoblach, Barbara

AU - Laborenz, Janina

AU - Boos, Felix

AU - Kowarzyk, Jacqueline

AU - Ben-Dor, Shifra

AU - Zalckvar, Einat

AU - Herrmann, Johannes M.

AU - Rachubinski, Richard A.

AU - Pines, Ophry

AU - Rapaport, Doron

AU - Michnick, Stephen W.

AU - Levy, Emmanuel D.

AU - Schuldiner, Maya

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

AB - Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of similar to 5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

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M3 - مقالة

C2 - 29988094

SN - 1548-7091

VL - 15

SP - 617

EP - 622

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Genome-wide SWAp-Tag yeast libraries for proteome exploration

Abstract

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