TY - JOUR
T1 - Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain
AU - Banerjee, Bodhisattwa
AU - Koner, Debaprasad
AU - Karasik, David
AU - Saha, Nirmalendu
N1 - Publisher Copyright: © 2020 Elsevier Inc.
PY - 2021/1
Y1 - 2021/1
N2 - Long non-coding RNAs (lncRNAs) are the master regulators of numerous biological processes. Hypoxia causes oxidative stress with severe and detrimental effects on brain function and acts as a critical initiating factor in the pathogenesis of Alzheimer's disease (AD). From the RNA-Seq in the forebrain (Fb), midbrain (Mb), and hindbrain (Hb) regions of hypoxic and normoxic zebrafish, we identified novel lncRNAs, whose potential cis targets showed involvement in neuronal development and differentiation pathways. Under hypoxia, several lncRNAs and mRNAs were differentially expressed. Co-expression studies indicated that the Fb and Hb regions' potential lncRNA target genes were involved in the AD pathogenesis. In contrast, those in Mb (cry1b, per1a, cipca) was responsible for regulating circadian rhythm. We identified specific lncRNAs present in the syntenic regions between zebrafish and humans, possibly functionally conserved. We thus identified several conserved lncRNAs as the probable regulators of AD genes (adrb3b, cav1, stat3, bace2, apoeb, psen1, s100b).
AB - Long non-coding RNAs (lncRNAs) are the master regulators of numerous biological processes. Hypoxia causes oxidative stress with severe and detrimental effects on brain function and acts as a critical initiating factor in the pathogenesis of Alzheimer's disease (AD). From the RNA-Seq in the forebrain (Fb), midbrain (Mb), and hindbrain (Hb) regions of hypoxic and normoxic zebrafish, we identified novel lncRNAs, whose potential cis targets showed involvement in neuronal development and differentiation pathways. Under hypoxia, several lncRNAs and mRNAs were differentially expressed. Co-expression studies indicated that the Fb and Hb regions' potential lncRNA target genes were involved in the AD pathogenesis. In contrast, those in Mb (cry1b, per1a, cipca) was responsible for regulating circadian rhythm. We identified specific lncRNAs present in the syntenic regions between zebrafish and humans, possibly functionally conserved. We thus identified several conserved lncRNAs as the probable regulators of AD genes (adrb3b, cav1, stat3, bace2, apoeb, psen1, s100b).
KW - Alzheimer's disease
KW - Brain regions
KW - Hypoxia
KW - Long non-coding RNAs
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85097190030&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.ygeno.2020.11.023
DO - https://doi.org/10.1016/j.ygeno.2020.11.023
M3 - مقالة
C2 - 33264657
SN - 0888-7543
VL - 113
SP - 29
EP - 43
JO - Genomics
JF - Genomics
IS - 1P1
ER -