Genome-wide identification of novel long non-coding RNAs and their possible roles in hypoxic zebrafish brain

Bodhisattwa Banerjee, Debaprasad Koner, David Karasik, Nirmalendu Saha

Research output: Contribution to journalArticlepeer-review

Abstract

Long non-coding RNAs (lncRNAs) are the master regulators of numerous biological processes. Hypoxia causes oxidative stress with severe and detrimental effects on brain function and acts as a critical initiating factor in the pathogenesis of Alzheimer's disease (AD). From the RNA-Seq in the forebrain (Fb), midbrain (Mb), and hindbrain (Hb) regions of hypoxic and normoxic zebrafish, we identified novel lncRNAs, whose potential cis targets showed involvement in neuronal development and differentiation pathways. Under hypoxia, several lncRNAs and mRNAs were differentially expressed. Co-expression studies indicated that the Fb and Hb regions' potential lncRNA target genes were involved in the AD pathogenesis. In contrast, those in Mb (cry1b, per1a, cipca) was responsible for regulating circadian rhythm. We identified specific lncRNAs present in the syntenic regions between zebrafish and humans, possibly functionally conserved. We thus identified several conserved lncRNAs as the probable regulators of AD genes (adrb3b, cav1, stat3, bace2, apoeb, psen1, s100b).

Original languageEnglish
Pages (from-to)29-43
Number of pages15
JournalGenomics
Volume113
Issue number1P1
DOIs
StatePublished - Jan 2021

Keywords

  • Alzheimer's disease
  • Brain regions
  • Hypoxia
  • Long non-coding RNAs
  • Zebrafish

All Science Journal Classification (ASJC) codes

  • Genetics

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