Gaining insight into membrane protein structure using isotope-edited FTIR

Joshua Manor, Isaiah T. Arkin

Research output: Contribution to journalReview articlepeer-review

Abstract

FTIR spectroscopy has long been used as a tool used to gain average structural information on proteins. With the advent of stable isotope editing, FTIR can be used to derive accurate information on isolated amino acids. In particular, in an anisotropic sample such as membrane layers, it is possible to measure the orientation of the peptidic carbonyl groups. Herein, we review the theory that enables one to obtain accurate restraints from FTIR spectroscopy, alongside considerations for sample suitability and general applicability. We also propose approaches that may be used to generate structural models of simple membrane proteins based on FTIR orientational restraints. This article is part of a Special Issue entitled: FTIR in membrane proteins and peptide studies.

Original languageAmerican English
Pages (from-to)2256-2264
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1828
Issue number10
DOIs
StatePublished - 2013

Keywords

  • FTIR
  • Isotope editing
  • Membrane protein
  • Molecular modeling

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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