Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Jeffrey Scott Fites; Neta Shlezinger; Tobias M. Hohl; Bruce S. Klein

doi:10.1007/978-1-0716-1182-1_7

Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Jeffrey Scott Fites, Neta Shlezinger, Tobias M. Hohl, Bruce S. Klein

The Koret School of Veterinary Medicine

The Hebrew University of Jerusalem

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	111-119
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-1182-1_7
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2260

Keywords

Fluorescent reporters
Host-Blastomyces interactions

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-1182-1_7

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abstract = "Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.",

keywords = "Fluorescent reporters, Host-Blastomyces interactions",

author = "Fites, \{Jeffrey Scott\} and Neta Shlezinger and Hohl, \{Tobias M.\} and Klein, \{Bruce S.\}",

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AU - Klein, Bruce S.

PY - 2021

Y1 - 2021

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AB - Fluorescence-based techniques enable researchers to monitor physiologic processes, specifically fungal cell viability and death, during cellular encounters with the mammalian immune system with single event resolution. By incorporating two independent fluorescent probes in fungal organisms either prior to, or ensuing experimental infection in mice or in cultured leukocytes, it is possible to distinguish and quantify live and killed fungal cells to interrogate genetic, pharmacologic, and cellular determinants that shape host–fungal cell outcomes. This chapter reviews the techniques and applications of fluorescent fungal reporters of viability, with emphasis on the North American endemic dimorphic fungus, Blastomyces dermatitidis.

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ER -

Fungal Bioreporters to Monitor Outcomes of Blastomyces: Host–Cell Interactions

Abstract

Publication series

Keywords

All Science Journal Classification (ASJC) codes

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