Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice

André P. Tittel; Christoph Heuser; Christina Ohliger; Chrystel Llanto; Simon Yona; Günter J. Hämmerling; Daniel R. Engel; Natalio Garbi; Christian Kurts

doi:https://doi.org/10.1038/nmeth.1905

Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice

André P. Tittel, Christoph Heuser, Christina Ohliger, Chrystel Llanto, Simon Yona, Günter J. Hämmerling, Daniel R. Engel, Natalio Garbi, Christian Kurts

Research output: Contribution to journal › Article › peer-review

Abstract

Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP) 1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c⁺ cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.

Original language	English
Pages (from-to)	385-390
Number of pages	6
Journal	Nature Methods
Volume	9
Issue number	4
DOIs	https://doi.org/10.1038/nmeth.1905
State	Published - Apr 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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@article{b546d27385734f8e924dfbb92bbd6d63,

title = "Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice",

abstract = "Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP) 1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c+ cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.",

author = "Tittel, {Andr{\'e} P.} and Christoph Heuser and Christina Ohliger and Chrystel Llanto and Simon Yona and H{\"a}mmerling, {G{\"u}nter J.} and Engel, {Daniel R.} and Natalio Garbi and Christian Kurts",

note = "Funding Information: We thank S. Jung for constructive discussions and correcting the manuscript, B. Schumak (University of Bonn) for providing CD11c.DOG mice, G. Kublbeck and S. Schmidt for assistance in the generation of CD11c.LuciDTR mice. We acknowledge support by the Central Animal Facilities of the Medical Faculty Bonn and the Flow Cytometry Core Facility at the Institutes of Molecular Medicine and Experimental Immunology Bonn. This work was supported by the Deutsche Forschungsgemeinschaft (Ku1038/5 and SFBTR57TP10 to C.K.) and EU Project NoE-MUGEN (LSHG-CT-2005-005203 to G.H.).",

year = "2012",

month = apr,

doi = "https://doi.org/10.1038/nmeth.1905",

language = "الإنجليزيّة",

volume = "9",

pages = "385--390",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Research",

number = "4",

}

TY - JOUR

T1 - Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice

AU - Tittel, André P.

AU - Heuser, Christoph

AU - Ohliger, Christina

AU - Llanto, Chrystel

AU - Yona, Simon

AU - Hämmerling, Günter J.

AU - Engel, Daniel R.

AU - Garbi, Natalio

AU - Kurts, Christian

N1 - Funding Information: We thank S. Jung for constructive discussions and correcting the manuscript, B. Schumak (University of Bonn) for providing CD11c.DOG mice, G. Kublbeck and S. Schmidt for assistance in the generation of CD11c.LuciDTR mice. We acknowledge support by the Central Animal Facilities of the Medical Faculty Bonn and the Flow Cytometry Core Facility at the Institutes of Molecular Medicine and Experimental Immunology Bonn. This work was supported by the Deutsche Forschungsgemeinschaft (Ku1038/5 and SFBTR57TP10 to C.K.) and EU Project NoE-MUGEN (LSHG-CT-2005-005203 to G.H.).

PY - 2012/4

Y1 - 2012/4

N2 - Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP) 1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c+ cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.

AB - Transgenic mice expressing the diphtheria toxin receptor (DTR) in specific cell types are key tools for functional studies in several biological systems. B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J (CD11c.DTR) and B6.Cg-Tg(Itgax-DTR/OVA/EGFP) 1Gjh/Crl (CD11c.DOG) mice express the DTR in CD11c+ cells, allowing conditional depletion of dendritic cells. We report that dendritic-cell depletion in these models caused polymorphonuclear neutrophil (PMN) release from the bone marrow, which caused chemokine-dependent neutrophilia after 6-24 h and increased bacterial clearance in a mouse pyelonephritis model. We present a transgenic mouse line, B6.Cg-Tg(Itgax-EGFP-CRE-DTR-LUC)2Gjh/Crl (CD11c.LuciDTR), which is unaffected by early neutrophilia. However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be considered in experimental settings where neutrophils may be involved.

UR - http://www.scopus.com/inward/record.url?scp=84859212675&partnerID=8YFLogxK

U2 - https://doi.org/10.1038/nmeth.1905

DO - https://doi.org/10.1038/nmeth.1905

M3 - مقالة

C2 - 22367054

SN - 1548-7091

VL - 9

SP - 385

EP - 390

JO - Nature Methods

JF - Nature Methods

IS - 4

ER -

Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice

Abstract

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