Functional photoacoustic microscopy of pH

Muhammad Rameez Chatni; Junjie Yao; Amos Danielli; Christopher P. Favazza; Konstantin I. Maslov; Lihong V. Wang

doi:10.1117/1.3644495

Functional photoacoustic microscopy of pH

Muhammad Rameez Chatni, Junjie Yao, Amos Danielli, Christopher P. Favazza, Konstantin I. Maslov, Lihong V. Wang

Research output: Contribution to journal › Article › peer-review

Abstract

pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.

Original language	English
Article number	100503
Journal	Journal of Biomedical Optics
Volume	16
Issue number	10
DOIs	https://doi.org/10.1117/1.3644495
State	Published - Oct 2011
Externally published	Yes

Keywords

SNARF-5F carboxylic acid
pH imaging
photoacoustic microscopy

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.3644495

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title = "Functional photoacoustic microscopy of pH",

abstract = "pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.",

keywords = "SNARF-5F carboxylic acid, pH imaging, photoacoustic microscopy",

author = "Chatni, {Muhammad Rameez} and Junjie Yao and Amos Danielli and Favazza, {Christopher P.} and Maslov, {Konstantin I.} and Wang, {Lihong V.}",

note = "Funding Information: The authors thank James Ballard and Sandra Matteucci for help in manuscript edition. This work was supported in part by National Institutes of Health Grant Nos. U54CA136398 and R01 EB000712.",

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doi = "10.1117/1.3644495",

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AU - Chatni, Muhammad Rameez

AU - Yao, Junjie

AU - Danielli, Amos

AU - Favazza, Christopher P.

AU - Maslov, Konstantin I.

AU - Wang, Lihong V.

N1 - Funding Information: The authors thank James Ballard and Sandra Matteucci for help in manuscript edition. This work was supported in part by National Institutes of Health Grant Nos. U54CA136398 and R01 EB000712.

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Y1 - 2011/10

N2 - pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.

AB - pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.

KW - SNARF-5F carboxylic acid

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KW - photoacoustic microscopy

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M3 - مقالة

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JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 10

M1 - 100503

ER -

Functional photoacoustic microscopy of pH

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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