Functional modeling of electrochemical whole-cell biosensors

Hadar Ben-Yoav; Alva Biran; Marek Sternheim; Shimshon Belkin; Amihay Freeman; Yosi Shacham-Diamand

doi:https://doi.org/10.1016/j.snb.2013.02.032

Functional modeling of electrochemical whole-cell biosensors

Hadar Ben-Yoav, Alva Biran, Marek Sternheim, Shimshon Belkin, Amihay Freeman, Yosi Shacham-Diamand

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.

Original language	American English
Pages (from-to)	479-485
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	181
DOIs	https://doi.org/10.1016/j.snb.2013.02.032
State	Published - 25 Mar 2013

Keywords

Biochips
Bioelectrochemistry
Michaelis-Menten kinetics
Modeling
Whole-cell biosensors

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

https://doi.org/10.1016/j.snb.2013.02.032

Cite this

@article{1f5b1b9de58c481787c6bf5c07a324c4,

title = "Functional modeling of electrochemical whole-cell biosensors",

abstract = "The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.",

keywords = "Biochips, Bioelectrochemistry, Michaelis-Menten kinetics, Modeling, Whole-cell biosensors",

author = "Hadar Ben-Yoav and Alva Biran and Marek Sternheim and Shimshon Belkin and Amihay Freeman and Yosi Shacham-Diamand",

note = "Funding Information: This work was partially supported by the “Dip-chip” project funded by the German–Israeli BMBF-MOST Cooperation in Water Technology grant number WT0601/02WU0844 . The work was also supported by the Tel Aviv University Scholarship Fund . We wish to thank to Dr. Sefi Vernick for the multiple discussions on the model development. We also wish to thank Dr. Rami Pedahzur and Dr. Sharon Yagur-Kroll for the fruitful discussion and for the development and providing of the genetically engineered E. coli whole-cell biosensors. We are grateful to Dr. Sebastian Buchinger and Dr. Georg Reifferscheid for the fruitful discussion on toxicity assays.",

year = "2013",

month = mar,

day = "25",

doi = "https://doi.org/10.1016/j.snb.2013.02.032",

language = "American English",

volume = "181",

pages = "479--485",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Functional modeling of electrochemical whole-cell biosensors

AU - Ben-Yoav, Hadar

AU - Biran, Alva

AU - Sternheim, Marek

AU - Belkin, Shimshon

AU - Freeman, Amihay

AU - Shacham-Diamand, Yosi

N1 - Funding Information: This work was partially supported by the “Dip-chip” project funded by the German–Israeli BMBF-MOST Cooperation in Water Technology grant number WT0601/02WU0844 . The work was also supported by the Tel Aviv University Scholarship Fund . We wish to thank to Dr. Sefi Vernick for the multiple discussions on the model development. We also wish to thank Dr. Rami Pedahzur and Dr. Sharon Yagur-Kroll for the fruitful discussion and for the development and providing of the genetically engineered E. coli whole-cell biosensors. We are grateful to Dr. Sebastian Buchinger and Dr. Georg Reifferscheid for the fruitful discussion on toxicity assays.

PY - 2013/3/25

Y1 - 2013/3/25

N2 - The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.

AB - The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.

KW - Biochips

KW - Bioelectrochemistry

KW - Michaelis-Menten kinetics

KW - Modeling

KW - Whole-cell biosensors

UR - http://www.scopus.com/inward/record.url?scp=84875179279&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.snb.2013.02.032

DO - https://doi.org/10.1016/j.snb.2013.02.032

M3 - Article

SN - 0925-4005

VL - 181

SP - 479

EP - 485

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Functional modeling of electrochemical whole-cell biosensors

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this