@article{deb1bd2cf3dd4f1dac5fcfd5e4c171f1,
title = "Functional labeling of neurons and their projections using the synthetic activity-dependent promoter E-SARE",
abstract = "Identifying the neuronal ensembles that respond to specific stimuli and mapping their projection patterns in living animals are fundamental challenges in neuroscience. To this end, we engineered a synthetic promoter, the enhanced synaptic activity-responsive element (E-SARE), that drives neuronal activity-dependent gene expression more potently than other existing immediate-early gene promoters. Expression of a drug-inducible Cre recombinase downstream of E-SARE enabled imaging of neuronal populations that respond to monocular visual stimulation and tracking of their long-distance thalamocortical projections in living mice. Targeted cell-attached recordings and calcium imaging of neurons in sensory cortices revealed that E-SARE reporter expression correlates with sensory-evoked neuronal activity at the single-cell level and is highly specific to the type of stimuli presented to the animals. This activity-dependent promoter can expand the repertoire of genetic approaches for high-resolution anatomical and functional analysis of neural circuits.",
author = "Takashi Kawashima and Kazuo Kitamura and Kanzo Suzuki and Mio Nonaka and Satoshi Kamijo and Sayaka Takemoto-Kimura and Masanobu Kano and Hiroyuki Okuno and Kenichi Ohki and Haruhiko Bito",
note = "We thank R. Kotin (US National Institutes of Health), B. Davidson (University of Iowa) and I. Bezprozvanny (University of Texas Southwestern Medical Center) for original AAV vectors; S. Josselyn and P. Frankland for the in vivo viral injection protocol; H. Kawasaki for advice on LGN experiments; V. Ramirez-Amaya for advice on Arc detection; S. Andreandis (The State University of New York, Buffalo) for a cHS4 construct; C. Cepko (Harvard University) for an ERT2CreERT2 construct; T. Curran (University of Pennsylvania) for a c-fos promoter construct; I. Imayoshi (Kyoto University) for a loxP-flanked STOP construct; L. Looger (Howard Hughes Medical Institute, Janelia Farm) for a GCaMP5G construct; A. Miyawaki (RIKEN BSI) for a Venus construct; H. Miyoshi (RIKEN BRC) for a lentiviral construct; the Research Support Center of the Graduate School of Medical Sciences at Kyushu University for technical support; and Carl Zeiss Japan for access to an LSM780. We thank all of the members of the Bito and Ohki laboratories for support and discussion. We are particularly indebted to Y. Kondo, K. Saiki, R. Gyobu and T. Kinbara for assistance. This work was supported in part by grants-in-aid from the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) and Japan Society for the Promotion of Science (JSPS) (to K.K., M.N., M.K., H.O. and H.B.), the Strategic Research Program for Brain Sciences (to M.K.) as well as grants from CREST–Japan Science and Technology Agency (JST) (to K.O. and H.B.), the Strategic International Research Cooperative Program Japan-Mexico (SICPME-JST, to H.B.) and the Mitsubishi Foundation (to H.B.). T.K., K.S., M. N. and S.K. were supported by JSPS fellowships.",
year = "2013",
month = sep,
doi = "https://doi.org/10.1038/NMETH.2559",
language = "الإنجليزيّة",
volume = "10",
pages = "889--895",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "9",
}