Functional classification of memory CD8 + T cells by CX 3 CR1 expression

Jan P. Boettcher; Marc Beyer; Felix Meissner; Zeinab Abdullah; Jil Sander; Bastian Hochst; Sarah Eickhoff; Jan C. Rieckmann; Caroline Russo; Tanja Bauer; Tobias Flecken; Dominik Giesen; Daniel Engel; Steffen Jung; Dirk H. Busch; Ulrike Protzer; Robert Thimme; Matthias Mann; Christian Kurts; Joachim L. Schultze; Wolfgang Kastenmuller; Percy A. Knolle

doi:10.1038/ncomms9306

Functional classification of memory CD8 + T cells by CX 3 CR1 expression

Jan P. Boettcher, Marc Beyer, Felix Meissner, Zeinab Abdullah, Jil Sander, Bastian Hochst, Sarah Eickhoff, Jan C. Rieckmann, Caroline Russo, Tanja Bauer, Tobias Flecken, Dominik Giesen, Daniel Engel, Steffen Jung, Dirk H. Busch, Ulrike Protzer, Robert Thimme, Matthias Mann, Christian Kurts, Joachim L. SchultzeWolfgang Kastenmuller, Percy A. Knolle

Department of Systems Immunology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Localization of memory CD8⁺ T cells to lymphoid or peripheral tissues is believed to correlate with proliferative capacity or effector function. Here we demonstrate that the fractalkine-receptor/CX 3 CR1 distinguishes memory CD8⁺ T cells with cytotoxic effector function from those with proliferative capacity, independent of tissue-homing properties. CX₃ CR1-based transcriptome and proteome-profiling defines a core signature of memory CD8⁺ T cells with effector function. We find CD62L hi CX³ CR1⁺ memory T cells that reside within lymph nodes. This population shows distinct migration patterns and positioning in proximity to pathogen entry sites. Virus-specific CX₃CR1⁺ memory CD8⁺ T cells are scarce during chronic infection in humans and mice but increase when infection is controlled spontaneously or by therapeutic intervention. This CX₃ CR1-based functional classification will help to resolve the principles of protective CD8⁺ T-cell memory.

Original language	English
Article number	8306
Number of pages	17
Journal	Nature Communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms9306
State	Published - 25 Sep 2015

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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Boettcher, J. P., Beyer, M., Meissner, F., Abdullah, Z., Sander, J., Hochst, B., Eickhoff, S., Rieckmann, J. C., Russo, C., Bauer, T., Flecken, T., Giesen, D., Engel, D., Jung, S., Busch, D. H., Protzer, U., Thimme, R., Mann, M., Kurts, C., ... Knolle, P. A. (2015). Functional classification of memory CD8 + T cells by CX 3 CR1 expression. Nature Communications, 6, Article 8306. https://doi.org/10.1038/ncomms9306

@article{2597158c5d6b4acfbaed34676fa956e0,

title = "Functional classification of memory CD8 + T cells by CX 3 CR1 expression",

abstract = "Localization of memory CD8+ T cells to lymphoid or peripheral tissues is believed to correlate with proliferative capacity or effector function. Here we demonstrate that the fractalkine-receptor/CX 3 CR1 distinguishes memory CD8+ T cells with cytotoxic effector function from those with proliferative capacity, independent of tissue-homing properties. CX3 CR1-based transcriptome and proteome-profiling defines a core signature of memory CD8+ T cells with effector function. We find CD62L hi CX3 CR1+ memory T cells that reside within lymph nodes. This population shows distinct migration patterns and positioning in proximity to pathogen entry sites. Virus-specific CX3CR1+ memory CD8+ T cells are scarce during chronic infection in humans and mice but increase when infection is controlled spontaneously or by therapeutic intervention. This CX3 CR1-based functional classification will help to resolve the principles of protective CD8+ T-cell memory.",

author = "Boettcher, \{Jan P.\} and Marc Beyer and Felix Meissner and Zeinab Abdullah and Jil Sander and Bastian Hochst and Sarah Eickhoff and Rieckmann, \{Jan C.\} and Caroline Russo and Tanja Bauer and Tobias Flecken and Dominik Giesen and Daniel Engel and Steffen Jung and Busch, \{Dirk H.\} and Ulrike Protzer and Robert Thimme and Matthias Mann and Christian Kurts and Schultze, \{Joachim L.\} and Wolfgang Kastenmuller and Knolle, \{Percy A.\}",

note = "We thank Elmar Endl and Andreas Dolf from the core facility flow cytometry and cell sorting for excellent technical assistance and the DFG SFB 704, SFB TR36 and SFB TR57 and the Excellence Cluster ImmunoSensation for their support. We thank Caetano Reis e Sousa, Immunobiology Laboratory, Francis Crick Institute, for critical reading of the manuscript. Contributions J.P.B., M.B., F.M., Z.A., B.H., S.E., J.C.R., C.R., T.B., T.F., D.G., D.E. performed the experiments and analysed the data; M.B., F.M., J.C.R., J.S. and J.L.S. performed the bioinformatic analyses; F.M. and J.C.R. performed and analysed the proteomics measurements; J.P.B., M.B., F.M., S.J., D.H.B., U.P., R.T., M.M., C.K., J.L.S., W.K. and P.K. designed the experiments and provided essential reagents as well as essential analysis technology; J.P.B., M.B., F.M., J.L.S., C.K., W.K. and P.A.K. wrote the manuscript.",

year = "2015",

month = sep,

day = "25",

doi = "10.1038/ncomms9306",

language = "الإنجليزيّة",

volume = "6",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

}

Boettcher, JP, Beyer, M, Meissner, F, Abdullah, Z, Sander, J, Hochst, B, Eickhoff, S, Rieckmann, JC, Russo, C, Bauer, T, Flecken, T, Giesen, D, Engel, D, Jung, S, Busch, DH, Protzer, U, Thimme, R, Mann, M, Kurts, C, Schultze, JL, Kastenmuller, W & Knolle, PA 2015, 'Functional classification of memory CD8 + T cells by CX 3 CR1 expression', Nature Communications, vol. 6, 8306. https://doi.org/10.1038/ncomms9306

TY - JOUR

T1 - Functional classification of memory CD8 + T cells by CX 3 CR1 expression

AU - Boettcher, Jan P.

AU - Beyer, Marc

AU - Meissner, Felix

AU - Abdullah, Zeinab

AU - Sander, Jil

AU - Hochst, Bastian

AU - Eickhoff, Sarah

AU - Rieckmann, Jan C.

AU - Russo, Caroline

AU - Bauer, Tanja

AU - Flecken, Tobias

AU - Giesen, Dominik

AU - Engel, Daniel

AU - Jung, Steffen

AU - Busch, Dirk H.

AU - Protzer, Ulrike

AU - Thimme, Robert

AU - Mann, Matthias

AU - Kurts, Christian

AU - Schultze, Joachim L.

AU - Kastenmuller, Wolfgang

AU - Knolle, Percy A.

N1 - We thank Elmar Endl and Andreas Dolf from the core facility flow cytometry and cell sorting for excellent technical assistance and the DFG SFB 704, SFB TR36 and SFB TR57 and the Excellence Cluster ImmunoSensation for their support. We thank Caetano Reis e Sousa, Immunobiology Laboratory, Francis Crick Institute, for critical reading of the manuscript. Contributions J.P.B., M.B., F.M., Z.A., B.H., S.E., J.C.R., C.R., T.B., T.F., D.G., D.E. performed the experiments and analysed the data; M.B., F.M., J.C.R., J.S. and J.L.S. performed the bioinformatic analyses; F.M. and J.C.R. performed and analysed the proteomics measurements; J.P.B., M.B., F.M., S.J., D.H.B., U.P., R.T., M.M., C.K., J.L.S., W.K. and P.K. designed the experiments and provided essential reagents as well as essential analysis technology; J.P.B., M.B., F.M., J.L.S., C.K., W.K. and P.A.K. wrote the manuscript.

PY - 2015/9/25

Y1 - 2015/9/25

N2 - Localization of memory CD8+ T cells to lymphoid or peripheral tissues is believed to correlate with proliferative capacity or effector function. Here we demonstrate that the fractalkine-receptor/CX 3 CR1 distinguishes memory CD8+ T cells with cytotoxic effector function from those with proliferative capacity, independent of tissue-homing properties. CX3 CR1-based transcriptome and proteome-profiling defines a core signature of memory CD8+ T cells with effector function. We find CD62L hi CX3 CR1+ memory T cells that reside within lymph nodes. This population shows distinct migration patterns and positioning in proximity to pathogen entry sites. Virus-specific CX3CR1+ memory CD8+ T cells are scarce during chronic infection in humans and mice but increase when infection is controlled spontaneously or by therapeutic intervention. This CX3 CR1-based functional classification will help to resolve the principles of protective CD8+ T-cell memory.

AB - Localization of memory CD8+ T cells to lymphoid or peripheral tissues is believed to correlate with proliferative capacity or effector function. Here we demonstrate that the fractalkine-receptor/CX 3 CR1 distinguishes memory CD8+ T cells with cytotoxic effector function from those with proliferative capacity, independent of tissue-homing properties. CX3 CR1-based transcriptome and proteome-profiling defines a core signature of memory CD8+ T cells with effector function. We find CD62L hi CX3 CR1+ memory T cells that reside within lymph nodes. This population shows distinct migration patterns and positioning in proximity to pathogen entry sites. Virus-specific CX3CR1+ memory CD8+ T cells are scarce during chronic infection in humans and mice but increase when infection is controlled spontaneously or by therapeutic intervention. This CX3 CR1-based functional classification will help to resolve the principles of protective CD8+ T-cell memory.

UR - http://www.scopus.com/inward/record.url?scp=84942693437&partnerID=8YFLogxK

U2 - 10.1038/ncomms9306

DO - 10.1038/ncomms9306

M3 - مقالة

SN - 2041-1723

VL - 6

JO - Nature Communications

JF - Nature Communications

M1 - 8306

ER -

Functional classification of memory CD8 + T cells by CX 3 CR1 expression

Abstract

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