Functional Assays Evaluating Immunosuppression Mediated by Myeloid-Derived Suppressor Cells

Or Reuven, Ivan Mikula, Hadas Ashkenazi-Preiser, Nira Twaik, Kerem Ben-Meir, Yaron Meirow, Leonor Daniel, Guy Kariv, Mahdi Kurd, Michal Baniyash

Research output: Contribution to journalArticlepeer-review


Myeloid-derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation, characterizing an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases, and induce immunosuppression. The suppressive effects of MDSCs on the immune system are studied mainly when focusing on their features, functions, and impact on target cells such as T cells, natural killer cells, and B cells, among others. Herein, we describe methods for the analysis of MDSC immunosuppressive features and functions, measuring different mediators that contribute to their activities and how they impact on T cell function. The protocols described are a continuation to those in a companion Current Protocols article by Reuven et al. (2022), which uses a generated single-cell suspension and isolated cells to test their activity.

Original languageAmerican English
Article numbere557
JournalCurrent Protocols
Issue number10
StatePublished - Oct 2022


  • MDSC
  • cell culture
  • chronic inflammation
  • flow cytometry
  • immunosuppression
  • inflammatory mouse model
  • myeloid-derived suppressor cell

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • General Pharmacology, Toxicology and Pharmaceutics
  • Medical Laboratory Technology
  • General Biochemistry,Genetics and Molecular Biology
  • Health Informatics
  • General Neuroscience


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