@inbook{d14253255c98438b934d3d5b6d544500,
title = "FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs",
abstract = "With the development of fluorescent proteins (FPs) and advanced optical microscopy techniques, F{\"o}rster or fluorescence resonance energy transfer (FRET) has become a powerful tool for real-time noninvasive visualization of a variety of biological processes, including kinase activities, with high spatiotemporal resolution in living cells and organisms. FRET can be detected in appropriately configured microscopes as changes in fluorescence intensity, lifetime, and anisotropy. Here, we describe the preparation of samples expressing FP-based FRET sensors for RhoA kinase, intensity- and lifetime-based FRET imaging, and postimaging data analysis.",
keywords = "FLIM-FRET, FRET, Fluorescence lifetime, Fluorescent protein, Rho GTPase, RhoA, Sensitized emission FRET",
author = "Bajar, {Bryce T.} and Xinmeng Guan and Amy Lam and Lin, {Michael Z.} and Ryohei Yasuda and Tal Laviv and Jun Chu",
note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2035-9_2",
language = "الإنجليزيّة",
isbn = "978-1-0716-2034-2",
series = "Methods in Molecular Biology",
pages = "31--43",
editor = "Chenbei Chang and Jianbo Wang",
booktitle = "Cell Polarity Signaling",
}