TY - JOUR
T1 - FORTIS
T2 - a live-cell assay to monitor AMPA receptors using pH-sensitive fluorescence tags
AU - Calleja-Felipe, María
AU - Wojtas, Magdalena Natalia
AU - Diaz-González, Marta
AU - Ciceri, Dalila
AU - Escribano, Raúl
AU - Ouro, Alberto
AU - Morales, Miguel
AU - Knafo, Shira
N1 - Publisher Copyright: © 2021, The Author(s).
PY - 2021/5/27
Y1 - 2021/5/27
N2 - The real-time live fluorescent monitoring of surface AMPA receptors (AMPARs) could open new opportunities for drug discovery and phenotypic screening concerning neuropsychiatric disorders. We have developed FORTIS, a tool based on pH sensitivity capable of detecting subtle changes in surface AMPARs at a neuronal population level. The expression of SEP-GluA1 or pHuji-GluA1 recombinant AMPAR subunits in mammalian neurons cultured in 96-well plates enables surface AMPARs to be monitored with a microplate reader. Thus, FORTIS can register rapid changes in surface AMPARs induced by drugs or genetic modifications without having to rely on conventional electrophysiology or imaging. By combining FORTIS with pharmacological manipulations, basal surface AMPARs, and plasticity-like changes can be monitored. We expect that employing FORTIS to screen for changes in surface AMPARs will accelerate both neuroscience research and drug discovery.
AB - The real-time live fluorescent monitoring of surface AMPA receptors (AMPARs) could open new opportunities for drug discovery and phenotypic screening concerning neuropsychiatric disorders. We have developed FORTIS, a tool based on pH sensitivity capable of detecting subtle changes in surface AMPARs at a neuronal population level. The expression of SEP-GluA1 or pHuji-GluA1 recombinant AMPAR subunits in mammalian neurons cultured in 96-well plates enables surface AMPARs to be monitored with a microplate reader. Thus, FORTIS can register rapid changes in surface AMPARs induced by drugs or genetic modifications without having to rely on conventional electrophysiology or imaging. By combining FORTIS with pharmacological manipulations, basal surface AMPARs, and plasticity-like changes can be monitored. We expect that employing FORTIS to screen for changes in surface AMPARs will accelerate both neuroscience research and drug discovery.
UR - http://www.scopus.com/inward/record.url?scp=85106997470&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41398-021-01457-w
DO - https://doi.org/10.1038/s41398-021-01457-w
M3 - Article
C2 - 34045447
SN - 2158-3188
VL - 11
JO - Translational Psychiatry
JF - Translational Psychiatry
IS - 1
M1 - 324
ER -