Fluorescence anisotropy to detect in vivo stimulus-induced changes in chemoreceptor packing

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The anisotropy of the fluorescence emitted from fluorescent proteins, such as yellow fluorescent protein (YFP), is sensitive to Homo-FRET between the proteins. This effect can be used to detect in vivo ligand-induced changes in packing or conformation of tagged chemoreceptors. Such measurements of clustered or dispersed core-signaling units revealed quantitative dose-dependent responses of these sensors.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages247-252
Number of pages6
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1729

Keywords

  • Chemoreceptors
  • Fluorescence anisotropy
  • Homo-FRET

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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