TY - JOUR
T1 - Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures
AU - Jia, Kun
AU - Adam, Pierre M.
AU - Marks, Robert S.
AU - Ionescu, Rodica E.
N1 - Funding Information: The authors thank the University of Technology of Troyes (UTT) for the Stratégique Program 2009–2012, the COST European action MP1302 NanoSpectroscopy the ANR program ANR-07-Nano-032 “NP/CL” for supplement of the optical set-up and the Region Champagne-Ardenne grants NANO’MAT for the electron microscopy characterization. Dr. Ionescu also thanks the OSEO innovation program for the financial support of the project « Système de procédé de detection d’espèces biologiques ou chimiques QCM structuré » 2012–2014. Prof. Marks acknowledges the NTU-HUJ-BGU Nanomaterials for Energy and Water Management Programme under the Campus for Research Excellence and Technological Enterprise (CREATE), that is supported by the National Research Foundation, Prime Minister's Office, Singapore. Kun Jia kindly appreciates the Chinese Scholarship Council for funding his Ph.D. scholarship in France. Publisher Copyright: © 2015 Elsevier B.V. All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic "hot spots", thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10-12 M.
AB - We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic "hot spots", thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10-12 M.
KW - Bacterial template
KW - Nanostructures
KW - SERS detection
KW - Trans-1;2-bis(4-pyridyl)ethylene
UR - http://www.scopus.com/inward/record.url?scp=84922488079&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.snb.2015.01.049
DO - https://doi.org/10.1016/j.snb.2015.01.049
M3 - Article
SN - 0925-4005
VL - 211
SP - 213
EP - 219
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -