Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures

Kun Jia; Pierre M. Adam; Robert S. Marks; Rodica E. Ionescu

doi:https://doi.org/10.1016/j.snb.2015.01.049

Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures

Kun Jia, Pierre M. Adam, Robert S. Marks, Rodica E. Ionescu

Department of Biotechnology Engineering

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic "hot spots", thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10^-12 M.

Original language	American English
Pages (from-to)	213-219
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	211
DOIs	https://doi.org/10.1016/j.snb.2015.01.049
State	Published - 1 Jan 2015

Keywords

Bacterial template
Nanostructures
SERS detection
Trans-1;2-bis(4-pyridyl)ethylene

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

https://doi.org/10.1016/j.snb.2015.01.049

Cite this

@article{71f3d919e28441dd90061b81eaeaad67,

title = "Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures",

abstract = "We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic {"}hot spots{"}, thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10-12 M.",

keywords = "Bacterial template, Nanostructures, SERS detection, Trans-1;2-bis(4-pyridyl)ethylene",

author = "Kun Jia and Adam, {Pierre M.} and Marks, {Robert S.} and Ionescu, {Rodica E.}",

note = "Funding Information: The authors thank the University of Technology of Troyes (UTT) for the Strat{\'e}gique Program 2009–2012, the COST European action MP1302 NanoSpectroscopy the ANR program ANR-07-Nano-032 “NP/CL” for supplement of the optical set-up and the Region Champagne-Ardenne grants NANO{\textquoteright}MAT for the electron microscopy characterization. Dr. Ionescu also thanks the OSEO innovation program for the financial support of the project « Syst{\`e}me de proc{\'e}d{\'e} de detection d{\textquoteright}esp{\`e}ces biologiques ou chimiques QCM structur{\'e} » 2012–2014. Prof. Marks acknowledges the NTU-HUJ-BGU Nanomaterials for Energy and Water Management Programme under the Campus for Research Excellence and Technological Enterprise (CREATE), that is supported by the National Research Foundation, Prime Minister's Office, Singapore. Kun Jia kindly appreciates the Chinese Scholarship Council for funding his Ph.D. scholarship in France. Publisher Copyright: {\textcopyright} 2015 Elsevier B.V. All rights reserved.",

year = "2015",

month = jan,

day = "1",

doi = "https://doi.org/10.1016/j.snb.2015.01.049",

language = "American English",

volume = "211",

pages = "213--219",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures

AU - Jia, Kun

AU - Adam, Pierre M.

AU - Marks, Robert S.

AU - Ionescu, Rodica E.

N1 - Funding Information: The authors thank the University of Technology of Troyes (UTT) for the Stratégique Program 2009–2012, the COST European action MP1302 NanoSpectroscopy the ANR program ANR-07-Nano-032 “NP/CL” for supplement of the optical set-up and the Region Champagne-Ardenne grants NANO’MAT for the electron microscopy characterization. Dr. Ionescu also thanks the OSEO innovation program for the financial support of the project « Système de procédé de detection d’espèces biologiques ou chimiques QCM structuré » 2012–2014. Prof. Marks acknowledges the NTU-HUJ-BGU Nanomaterials for Energy and Water Management Programme under the Campus for Research Excellence and Technological Enterprise (CREATE), that is supported by the National Research Foundation, Prime Minister's Office, Singapore. Kun Jia kindly appreciates the Chinese Scholarship Council for funding his Ph.D. scholarship in France. Publisher Copyright: © 2015 Elsevier B.V. All rights reserved.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic "hot spots", thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10-12 M.

AB - We show herein for the first time the ability to fabricate highly sensitive SERS substrates using fixed Escherichia coli bacterial cells, used as templates after glutaraldehyde fixing, whereupon stable gold nanostructures of a suitable size for SERS generation were found lying on top of bacteria after an evaporation deposition procedure, followed by high temperature annealing. Thanks to the template effect provided by the bacterial cells, gold nanoparticles had been produced forming controlled agglomerate patterns containing high density plasmonic "hot spots", thereby contributing to a remarkable enhancement of Raman signals. Moreover, a TEM copper grid marker used during the gold evaporation process, enabled to precisely correlate the optical properties (including LSPR and SERS spectra) and SEM morphology of a pre-defined area of gold nanostructures. It was observed that modulating either gold film thickness or annealing temperature, enabled to influence the LSPR properties of the produced Au NPs, and this was demonstrated by obtaining a highly sensitive SERS detection of a model compound, trans-1,2-bis(4-pyridyl)ethylene (BPE), down to 10-12 M.

KW - Bacterial template

KW - Nanostructures

KW - SERS detection

KW - Trans-1;2-bis(4-pyridyl)ethylene

UR - http://www.scopus.com/inward/record.url?scp=84922488079&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.snb.2015.01.049

DO - https://doi.org/10.1016/j.snb.2015.01.049

M3 - Article

SN - 0925-4005

VL - 211

SP - 213

EP - 219

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Fixed Escherichia coli bacterial templates enable the production of sensitive SERS-based gold nanostructures

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this