Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium

Annie Vardanian, Eyal Kurzbaum, Yair Farber, Monica Butnariu, Robert Armon

Research output: Contribution to journalArticlepeer-review

Abstract

Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies’ count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.

Original languageAmerican English
Pages (from-to)401-404
Number of pages4
JournalFolia Microbiologica
Volume63
Issue number3
DOIs
StatePublished - 1 May 2018

All Science Journal Classification (ASJC) codes

  • Microbiology

Fingerprint

Dive into the research topics of 'Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium'. Together they form a unique fingerprint.

Cite this