TY - CHAP
T1 - Fabrication of Nanoscale Arrays to Study the Effect of Ligand Arrangement on Inhibitory Signaling in NK Cells
AU - Le Saux, Guillaume
AU - Toledo-Ashkenazi, Esti
AU - Schvartzman, Mark
N1 - Funding Information: This work was supported by Israel Science Foundation, Individual Grant No. 1401/15, and Israel Science Foundations: F.I.R.S.T. Individual Grant No. 2058/18. Publisher Copyright: © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Molecular scale nanopatterns of bioactive molecules have been used to study the effect of transmembrane receptor arrangement on a variety of cell types, including immune cells and their immune response in particular. However, state-of-the-art fabrication approaches have thus far enabled the production of patterns with control over one receptor type only. Herein, we describe a protocol to fabricate arrays for the molecular scale control of the segregation between activating and inhibitory receptors in NK cells. We used this platform to study how ligand segregation regulates NK cell inhibitory signaling and function. The arrays are based on patterns of nanodots of two metals, selectively functionalized with activating and inhibitory ligands. Due to the versatility of our functionalization approach, this protocol can be applied to configurate virtually any combination of extracellular ligands into controlled multifunctional arrays.
AB - Molecular scale nanopatterns of bioactive molecules have been used to study the effect of transmembrane receptor arrangement on a variety of cell types, including immune cells and their immune response in particular. However, state-of-the-art fabrication approaches have thus far enabled the production of patterns with control over one receptor type only. Herein, we describe a protocol to fabricate arrays for the molecular scale control of the segregation between activating and inhibitory receptors in NK cells. We used this platform to study how ligand segregation regulates NK cell inhibitory signaling and function. The arrays are based on patterns of nanodots of two metals, selectively functionalized with activating and inhibitory ligands. Due to the versatility of our functionalization approach, this protocol can be applied to configurate virtually any combination of extracellular ligands into controlled multifunctional arrays.
KW - Biofunctionalization
KW - Inhibition
KW - Nanolithography
KW - Natural killer cells
KW - Spatial control of receptors
UR - http://www.scopus.com/inward/record.url?scp=85158149798&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/978-1-0716-3135-5_20
DO - https://doi.org/10.1007/978-1-0716-3135-5_20
M3 - Chapter
C2 - 37106191
T3 - Methods in Molecular Biology
SP - 313
EP - 325
BT - Methods in Molecular Biology
ER -