TY - JOUR
T1 - Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers
AU - Sadhu, Raj Kumar
AU - Hernandez-Padilla, Christian
AU - Eisenbach, Yael Eshed
AU - Penič, Samo
AU - Zhang, Lixia
AU - Vishwasrao, Harshad D.
AU - Behkam, Bahareh
AU - Konstantopoulos, Konstantinos
AU - Shroff, Hari
AU - Iglič, Aleš
AU - Peles, Elior
AU - Nain, Amrinder S.
AU - Gov, Nir S.
N1 - Publisher Copyright: © 2023, Springer Nature Limited.
PY - 2023/9/12
Y1 - 2023/9/12
N2 - Protrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using Dual-View light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon.
AB - Protrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using Dual-View light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon.
UR - http://www.scopus.com/inward/record.url?scp=85170669526&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s41467-023-41273-y
DO - https://doi.org/10.1038/s41467-023-41273-y
M3 - مقالة
C2 - 37699891
SN - 2041-1723
VL - 14
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 5612
ER -