@article{03c60f0d8ad045d68eaf7d3786b7d8f1,
title = "Expansion microscopy of zebrafish for neuroscience and developmental biology studies",
abstract = "Expansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility of ExM in the larval and embryonic zebrafish, an important model organism for the study of neuroscience and development. Regarding neuroscience, we found that ExM enabled the tracing of fine processes of radial glia, which are not resolvable with diffraction-limited microscopy. ExM further resolved putative synaptic connections, as well as molecular differences between densely packed synapses. Finally, ExM could resolve subsynaptic protein organization, such as ring-like structures composed of glycine receptors. Regarding development, we used ExM to characterize the shapes of nuclear invaginations and channels, and to visualize cytoskeletal proteins nearby. We detected nuclear invagination channels at late prophase and telophase, potentially suggesting roles for such channels in cell division. Thus, ExM of the larval and embryonic zebrafish may enable systematic studies of how molecular components are configured in multiple contexts of interest to neuroscience and developmental biology.",
keywords = "Brain, Microscopy, Superresolution, Zebrafish",
author = "Limor Freifeld and Iris Odstrcil and Dominique F{\"o}rster and Alyson Ramirez and Gagnon, {James A.} and Owen Randlett and Costa, {Emma K.} and Shoh Asano and Celiker, {Orhan T.} and Ruixuan Gao and Martin-Alarcon, {Daniel A.} and Paul Reginato and Cortni Dick and Linlin Chen and David Schoppik and Florian Engert and Herwig Baier and Boyden, {Edward S.}",
note = "Funding Information: We thank Alex Schier for his support of A.R., J.A.G., and O.R., and for helpful discussions of the paper. We also thank Iain Cheeseman for useful comments on the paper. We thank Dr. Avi Jacobs for his help with STED imaging, Dr. Eliza Vasile for her help with SIM imaging, and Dr. Alexandra Lichtenstein and Dr. Miriam Shaharabani for their help with confocal microscopy. We thank Daniel M. Estandian and Giovanni Talei Franzesi for their help in larvae preparation for validation experiments. We thank Dr. Natalie Elia and Dr. Dikla Nachmias for their help with SIM imaging in preliminary validation experiments. We thank Yongxin Zhao and Fei Chen for their advice and code for validation data analysis. We thank Dr. Pablo Blinder for use of his laboratory facilities for validation experiments described in SI Appendix, Fig. S1 B and D. L.F. was supported by a postdoctoral fellowship from the Simons Center for the Social Brain at the Massachusetts Institute of Technology (MIT). D.F. was supported by a European Molecular Biology Organization fellowship (ALTF 104-2013). A.R. is a Howard Hughes Medical Institute Gilliam Fellow. E.K.C. was funded by a MIT Peter J. Eloranta Summer Undergraduate Research Fellowship. D.A.M.-A. was funded by a McGovern Institute Shelly Razin Fund Fellowship. This work was funded, in part, by the Max Planck Society. E.S.B. was funded by the Howard Hughes Medical Institute-Simons Faculty Scholars Program, the Open Philanthropy Project, the MIT Media Laboratory, the New York Stem Cell Foundation, NIH Grant 1R01EB024261, NIH Grant 1R01MH110932, NIH Grant 2R01DA029639, NIH Grant 1R01NS087950, NIH Grant 1U01MH106011, John Doerr, and Jeremy and Joyce Wertheimer. Funding Information: ACKNOWLEDGMENTS. We thank Alex Schier for his support of A.R., J.A.G., and O.R., and for helpful discussions of the paper. We also thank Iain Cheeseman for useful comments on the paper. We thank Dr. Avi Jacobs for his help with STED imaging, Dr. Eliza Vasile for her help with SIM imaging, and Dr. Alexandra Lichtenstein and Dr. Miriam Shaharabani for their help with confocal microscopy. We thank Daniel M. Estandian and Giovanni Talei Franzesi for their help in larvae preparation for validation experiments. We thank Dr. Natalie Elia and Dr. Dikla Nachmias for their help with SIM imaging in preliminary validation experiments. We thank Yongxin Zhao and Fei Chen for their advice and code for validation data analysis. We thank Dr. Pablo Blinder for use of his laboratory facilities for validation experiments described in SI Appendix, Fig. S1 B and D. L.F. was supported by a postdoctoral fellowship from the Simons Center for the Social Brain at the Massachusetts Institute of Technology (MIT). D.F. was supported by a European Molecular Biology Organization fellowship (ALTF 104-2013). A.R. is a Howard Hughes Medical Institute Gilliam Fellow. E.K.C. was funded by a MIT Peter J. Eloranta Summer Undergraduate Research Fellowship. D.A.M.-A. was funded by a McGovern Institute Shelly Razin Fund Fellowship. This work was funded, in part, by the Max Planck Society. E.S.B. was funded by the Howard Hughes Medical Institute-Simons Faculty Scholars Program, the Open Philanthropy Project, the MIT Media Laboratory, the New York Stem Cell Foundation, NIH Grant 1R01EB024261, NIH Grant 1R01MH110932, NIH Grant 2R01DA029639, NIH Grant 1R01NS087950, NIH Grant 1U01MH106011, John Doerr, and Jeremy and Joyce Wertheimer.",
year = "2017",
month = dec,
day = "12",
doi = "https://doi.org/10.1073/pnas.1706281114",
language = "الإنجليزيّة",
volume = "114",
pages = "E10799--E10808",
number = "50",
}