Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

Ory Perelsman; Shoh Asano; Limor Freifeld

doi:https://doi.org/10.1007/978-1-0716-2051-9_13

Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

Ory Perelsman, Shoh Asano, Limor Freifeld

Biomedical Engineering

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.

Original language	English
Pages (from-to)	211-222
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2440
DOIs	https://doi.org/10.1007/978-1-0716-2051-9_13
State	Published - 2022

Keywords

Development
Expansion microscopy
Neuroscience
Superresolution microscopy
Zebrafish

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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https://doi.org/10.1007/978-1-0716-2051-9_13

Cite this

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title = "Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos",

abstract = "Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.",

keywords = "Development, Expansion microscopy, Neuroscience, Superresolution microscopy, Zebrafish",

author = "Ory Perelsman and Shoh Asano and Limor Freifeld",

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KW - Development

KW - Expansion microscopy

KW - Neuroscience

KW - Superresolution microscopy

KW - Zebrafish

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DO - https://doi.org/10.1007/978-1-0716-2051-9_13

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JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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