Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos

Ory Perelsman, Shoh Asano, Limor Freifeld

Research output: Contribution to journalArticlepeer-review


Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.

Original languageEnglish
Pages (from-to)211-222
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2022


  • Development
  • Expansion microscopy
  • Neuroscience
  • Superresolution microscopy
  • Zebrafish

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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