TY - JOUR
T1 - Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos
AU - Perelsman, Ory
AU - Asano, Shoh
AU - Freifeld, Limor
N1 - Publisher Copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
AB - Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
KW - Development
KW - Expansion microscopy
KW - Neuroscience
KW - Superresolution microscopy
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85125549706&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/978-1-0716-2051-9_13
DO - https://doi.org/10.1007/978-1-0716-2051-9_13
M3 - مقالة
C2 - 35218542
SN - 1064-3745
VL - 2440
SP - 211
EP - 222
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -