Abstract
Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human skin samples treated with fluorescent nanoparticles. The method is based on horizontal cryosections of skin samples, followed by confocal microscopy and image analysis. This protocol is relatively simple to perform with basic histological tools, thus it can serve for various dermatology assays.
| Original language | English |
|---|---|
| Article number | e4375 |
| Journal | BIO-PROTOCOL |
| Volume | 12 |
| Issue number | 7 |
| DOIs | |
| State | Published - 5 Apr 2022 |
Keywords
- Confocal microscopy
- Fluorescent nanoparticles
- Horizontal cryosectioning
- Nanoparticles penetration depth
- Skin permeability
All Science Journal Classification (ASJC) codes
- General Neuroscience
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology
- Plant Science
Fingerprint
Dive into the research topics of 'Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver